| Title: | NanoLC-EI-MS: Perspectives in Biochemical Analysis |
| Author(s): | Pereira Dos Santos NG; Maciel EVS; Vargas Medina DA; Lancas FM; |
| Address: | "Institute of Chemistry of Sao Carlos, University of Sao Paulo, Sao Carlos 13566-590, Brazil" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Although LC-MS with atmospheric pressure ionization (API) sources is the primary technique used in modern bioanalytical studies, electron ionization mass spectrometry (EI-MS) can provide some substantial advantages over it. EI-MS is a matrix effect-free technique that provides reproducible and comparable mass spectra, serving as a compound fingerprint for easy identification through automated comparison with spectral libraries. Leveraging EI-MS in biochemical studies can yield critical analytical benefits for targeted and untargeted analyses. However, to fully utilize EI-MS for heavy and non-volatile molecules, a new technology that enables the coupling of liquid chromatography with EI-MS is needed. Recent advancements in nanoLC have addressed the compatibility issues between LC and EI-MS, and innovative interfacing strategies such as Direct-EI, liquid electron ionization (LEI), and Cold-EI have extended the application of EI-MS beyond the determination of volatile organic molecules. This review provides an overview of the latest developments in nanoLC-EI-MS interfacing technologies, discussing their scope and limitations. Additionally, selected examples of nanoLC-EI-MS applications in the field of biochemical analysis are presented, highlighting the potential prospects and benefits that the establishment of this technique can bring to this field" |
| Keywords: | "*Spectrometry, Mass, Electrospray Ionization/methods *Electrons Chromatography, Liquid/methods Atmospheric Pressure Technology biochemical analysis electron ionization mass spectrometry metabolomics nano-liquid chromatography;" |
| Notes: | "MedlinePereira Dos Santos, Natalia Gabrielly Maciel, Edvaldo Vasconcelos Soares Vargas Medina, Deyber Arley Lancas, Fernando Mauro eng 2019/22724-7 2017/02147-0, 2019/26263-4, 2017/02147-0; 2017/21984-0 - EMU; 2017/21985-6 - EMU/Sao Paulo Research Foundation/ 307293/2014-9 and 308843/2019-3/National Council for Scientific and Technological Development/ Finance Code 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ Review Switzerland 2023/07/29 Int J Mol Sci. 2023 Jul 21; 24(14):11746. doi: 10.3390/ijms241411746" |
