| Title: | CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis) |
| Author(s): | Ono E; Handa T; Koeduka T; Toyonaga H; Tawfik MM; Shiraishi A; Murata J; Matsui K; |
| Address: | "Research Institute, Suntory Global Innovation Center Ltd, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: eiichiro_ono@suntory.co.jp. Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: r038ge@yamaguchi-u.ac.jp. Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: takaori@yamaguchi-u.ac.jp. Research Institute, Suntory Global Innovation Center Ltd, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: hiromi_toyonaga@skal.suntory.co.jp. Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: t014uj@yamaguchi-u.ac.jp. Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: shiraishi@sunbor.or.jp. Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1 Seika-dai, Seika, Soraku, Kyoto 619-0238, Japan. Electronic address: murata-j@sunbor.or.jp. Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan. Electronic address: matsui@yamaguchi-u.ac.jp" |
| DOI: | 10.1016/j.plaphy.2015.11.016 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Green leaf volatiles (GLVs) are C6-aliphatic aldehydes/alcohols/acetates, and biosynthesized from the central precursor fatty acid 13-hydroperoxides by 13-hydroperoxide lyases (HPLs) in various plant species. While GLVs have been implicated as defense compounds in plants, GLVs give characteristic grassy note to a bouquet of aroma in green tea, which is manufactured from young leaves of Camellia sinensis. Here we identify three HPL-related genes from C. sinensis via RNA-Sequencing (RNA-Seq) in silico, and functionally characterized a candidate gene, CYP74B24, as a gene encoding tea HPL. Recombinant CYP74B24 protein heterologously expressed in Escherichia coli specifically produced (Z)-3-hexenal from 13-HPOT with the optimal pH 6.0 in vitro. CYP74B24 gene was expressed throughout the aerial organs in a rather constitutive manner and further induced by mechanical wounding. Constitutive expression of CYP74B24 gene in intact tea leaves might account for low but substantial and constitutive formation of a subset of GLVs, some of which are stored as glycosides. Our results not only provide novel insights into the biological roles that GLVs play in tea plants, but also serve as basis for the improvement of aroma quality in tea manufacturing processes" |
| Keywords: | Acetates/metabolism Aldehyde-Lyases/genetics/*metabolism Aldehydes/metabolism Amino Acid Sequence Camellia sinensis/chemistry/*enzymology/genetics Cytochrome P-450 Enzyme System/genetics/*metabolism Gene Expression Linolenic Acids/metabolism Lipid Peroxid; |
| Notes: | "MedlineOno, Eiichiro Handa, Taiki Koeduka, Takao Toyonaga, Hiromi Tawfik, Moataz M Shiraishi, Akira Murata, Jun Matsui, Kenji eng Research Support, Non-U.S. Gov't France 2015/12/22 Plant Physiol Biochem. 2016 Jan; 98:112-8. doi: 10.1016/j.plaphy.2015.11.016. Epub 2015 Nov 27" |
