« Previous AbstractFlooding and Herbivory Interact to Alter Volatile Organic Compound Emissions in Two Maize Hybrids    Next AbstractDrought and flooding have distinct effects on herbivore-induced responses and resistance in Solanum dulcamara »

Proc Natl Acad Sci U S A

Title:		Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant
Author(s):		Nguyen CT; Kurenda A; Stolz S; Chetelat A; Farmer EE;
Address:		"Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, Switzerland. Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, Switzerland edward.farmer@unil.ch"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2018
Volume:		20180918
Issue:		40
Page Number:		10178 - 10183
DOI:		10.1073/pnas.1807049115
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In Arabidopsis, wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both of these spatially separated cell types strongly attenuated leaf-to-leaf electrical signaling. glr3.3 mutants were also compromised in their defense against herbivores. Since wounding is known to cause increases in cytosolic calcium, we monitored electrical signals and Ca(2+) transients simultaneously. This revealed that wound-induced membrane depolarizations in the wild-type preceded cytosolic Ca(2+) maxima. The axial and radial distributions of calcium fluxes were differentially affected in each glr mutant. Resolving a debate over which cell types are necessary for electrical signaling between leaves, we show that phloem sieve elements and xylem contact cells function together in this process"
Keywords:		Arabidopsis/*metabolism *Calcium Signaling *Membrane Potentials *Plant Diseases Plant Leaves/*metabolism Ricca's factor calcium jasmonate phloem xylem;
Notes:		"MedlineNguyen, Chi Tam Kurenda, Andrzej Stolz, Stephanie Chetelat, Aurore Farmer, Edward E eng Research Support, Non-U.S. Gov't 2018/09/20 Proc Natl Acad Sci U S A. 2018 Oct 2; 115(40):10178-10183. doi: 10.1073/pnas.1807049115. Epub 2018 Sep 18"