« Previous AbstractEvaluation of Resistance Management for the Oriental Fruit Moth (Lepidoptera: Tortricidae) to Insecticides in Brazilian Apple Orchards    Next Abstract"Evaluation of the chemical, sensory and volatile composition of sapota-do-Solimoes pulp at different ripening stages" »

J Cell Mol Med

Title:		GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients
Author(s):		Monteiro M; Moreira N; Pinto J; Pires-Luis AS; Henrique R; Jeronimo C; Bastos ML; Gil AM; Carvalho M; Guedes de Pinho P;
Address:		"UCIBIO@REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Porto, Portugal. Cancer Biology & Epigenetics Group, Portuguese Oncology Institute of Porto (IPOPorto), Porto, Portugal. Department of Pathology, Portuguese Oncology Institute of Porto (IPOPorto), Porto, Portugal. Department of Pathology and Molecular Immunology-Biomedical Sciences Institute (ICBAS), University of Porto, Porto, Portugal. CICECO-Instituto de Materiais de Aveiro (CICECO/UA), Departamento de Quimica, Universidade de Aveiro, Aveiro, Portugal. UFP Energy, Environment and Health Research Unit (FP-ENAS), Universidade Fernando Pessoa, Porto, Portugal"
Journal Title:		J Cell Mol Med
Year:		2017
Volume:		20170404
Issue:		9
Page Number:		2092 - 2105
DOI:		10.1111/jcmm.13132
ISSN/ISBN:		1582-4934 (Electronic) 1582-1838 (Print) 1582-1838 (Linking)
Abstract:		"The analysis of volatile organic compounds (VOCs) emanating from biological samples appears as one of the most promising approaches in metabolomics for the study of diseases, namely cancer. In fact, it offers advantages, such as non-invasiveness and robustness for high-throughput applications. The purpose of this work was to study the urinary volatile metabolic profile of patients with renal cell carcinoma (RCC) (n = 30) and controls (n = 37) with the aim of identifying a potential specific urinary volatile pattern as a non-invasive strategy to detect RCC. Moreover, the effect of some confounding factors such as age, gender, smoking habits and body mass index was evaluated as well as the ability of urinary VOCs to discriminate RCC subtypes and stages. A headspace solid-phase microextraction/gas chromatography-mass spectrometry-based method was performed, followed by multivariate data analysis. A variable selection method was applied to reduce the impact of potential redundant and noisy chromatographic variables, and all models were validated by Monte Carlo cross-validation and permutation tests. Regarding the effect of RCC on the urine VOCs composition, a panel of 21 VOCs descriptive of RCC was defined, capable of discriminating RCC patients from controls in principal component analysis. Discriminant VOCs were further individually validated in two independent samples sets (nine RCC patients and 12 controls, seven RCC patients with diabetes mellitus type 2) by univariate statistical analysis. Two VOCs were found consistently and significantly altered between RCC and controls (2-oxopropanal and, according to identification using NIST14, 2,5,8-trimethyl-1,2,3,4-tetrahydronaphthalene-1-ol), strongly suggesting enhanced potential as RCC biomarkers. Gender, smoking habits and body mass index showed negligible and age-only minimal effects on the urinary VOCs, compared to the deviations resultant from the disease. Moreover, in this cohort, the urinary volatilome did not show ability to discriminate RCC stages and histological subtypes. The results validated the value of urinary volatilome for the detection of RCC and advanced with the identification of potential RCC urinary biomarkers"
Keywords:		"Adult Aged Aged, 80 and over Biomarkers, Tumor/*urine Carcinoma, Renal Cell/*urine Case-Control Studies Discriminant Analysis Female *Gas Chromatography-Mass Spectrometry Humans Kidney Neoplasms/*urine Least-Squares Analysis Male *Metabolomics Middle Aged;"
Notes:		"MedlineMonteiro, Marcia Moreira, Nathalie Pinto, Joana Pires-Luis, Ana S Henrique, Rui Jeronimo, Carmen Bastos, Maria de Lourdes Gil, Ana M Carvalho, Marcia Guedes de Pinho, Paula eng Research Support, Non-U.S. Gov't England 2017/04/06 J Cell Mol Med. 2017 Sep; 21(9):2092-2105. doi: 10.1111/jcmm.13132. Epub 2017 Apr 4"