| Title: | "Chemical Composition, Antipathogenic and Cytotoxic Activity of the Essential Oil Extracted from Amorpha fruticosa Fruits" |
| Author(s): | Marinas IC; Oprea E; Buleandra M; Badea IA; Tihauan BM; Marutescu L; Angheloiu M; Matei E; Chifiriuc MC; |
| Address: | "Department of Botany and Microbiology, Faculty of Biology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, 90-92 Panduri Street, 050663 Bucharest, Romania. National Institute of Research & Development for Food Bioresources-IBA Bucharest, 5th Ancuta Baneasa Street, 020323 Bucharest, Romania. Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Street, 050663 Bucharest, Romania. Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Street, 050663 Bucharest, Romania. National Institute of Materials Physics-Magurele, 405A Atomistilor Street, 077125 Magurele, Romania. Academy of Romanian Scientists, 3rd Ilfov Street, 051157 Bucharest, Romania" |
| DOI: | 10.3390/molecules26113146 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The purpose of this paper was to characterize and investigate the antimicrobial potential of Amorpha fruticosa fruits essential oil (EO). The EO was extracted by hydrodistillation, analyzed by GC-MS, and then evaluated for its interaction with microbial and mammalian cells. The antimicrobial activity was assessed against bacterial and fungal strains, in a planktonic and adherent growth state, using qualitative and quantitative assays. The main components identified in A. fruticosa fruits EO were delta-cadinene, gamma-muurolene, and alpha-muurolene. The Gram-positive strains proved to be more susceptible than Gram-negative bacteria and fungal strains. The EO exhibited good antibiofilm activity, inhibiting the microbial adherence to the inert (96-well plates and Foley catheter section) and cellular substrata. The flow cytometry analysis revealed as one of the possible mechanisms of antimicrobial action the alteration of cell membrane hydrophobicity. The cytotoxicity on the L929 cell line occurred at concentrations higher than 0.3 mg/mL. Taken together, our results demonstrate that A. fruticosa fruits EO contains active compounds with selective inhibitory effect on different microbial strains in planktonic and biofilm growth state, explained at least partially by the interference with microbial membranes due to their hydrophobic character" |
| Keywords: | "Drug Screening Assays, Antitumor Fabaceae/*chemistry Humans Hydrophobic and Hydrophilic Interactions Microbial Sensitivity Tests Oils, Volatile/*chemistry/*pharmacology Gc-ms antibiofilm activity antimicrobial activity cytotoxicity essential oil flow cyto;" |
| Notes: | "MedlineMarinas, Ioana Cristina Oprea, Eliza Buleandra, Mihaela Badea, Irinel Adriana Tihauan, Bianca Maria Marutescu, Luminita Angheloiu, Marin Matei, Elena Chifiriuc, Mariana Carmen eng ICUB20968/30.12.2020/Universitatea din Bucuresti/ contract no. 21N/08.02.2019/Core programe project PN19-03/ Switzerland 2021/06/03 Molecules. 2021 May 24; 26(11):3146. doi: 10.3390/molecules26113146" |
