| Title: | Biomonitoring of workers using nuclear magnetic resonance-based metabolomics of exhaled breath condensate: A pilot study |
| Author(s): | Maniscalco M; Paris D; Melck D; Chiariello N; Di Napoli F; Manno M; Iavicoli I; Motta A; |
| Address: | "Pulmonary Rehabilitation Unit, ICS Maugeri SpA SB, Institute of Telese Terme, Benevento, Italy. Electronic address: mauromaniscalco@hotmail.com. Institute of Biomolecular Chemistry, National Research Council, Pozzuoli, Naples, Italy. Oerlikon Friction System (Italia) s.r.l., Localita Pascarola, Caivano, Naples, Italy. Section of Occupational Medicine, Department of Public Health, University of Naples, Naples, Italy. Institute of Biomolecular Chemistry, National Research Council, Pozzuoli, Naples, Italy. Electronic address: andrea.motta@icb.cnr.it" |
| DOI: | 10.1016/j.toxlet.2018.10.018 |
| ISSN/ISBN: | 1879-3169 (Electronic) 0378-4274 (Linking) |
| Abstract: | "Exhaled breath condensate (EBC) is a non-invasive biological matrix that allows easy access to the lung epithelial lining fluid. It can provide, for instance, useful biochemical information in workers following inhalatory exposure. Recently, nuclear magnetic resonance (NMR)-based metabolomics has been applied to EBC profiling to identify metabolic phenotypes ('metabotypes') of relevance in respiratory medicine. We aimed at verifying if NMR-based metabolomics of EBC, combined with statistical analysis, could find differences in metabolomic profiles between groups of subjects occupationally exposed to levels of airborne inhalable dust, phenol, formaldehyde and volatile organic compounds (VOCs) below regulatory limits. Samples were collected from 20 blue-collar workers without wearing a mask (therefore 'exposed' to ambient air) and after wearing a mask ('not exposed'). This procedure was only allowed after the standard air quality tests had shown levels of airborne xenobiotics below the occupational exposure limit or even below the LOD. Ten white-collar controls were also included. After partial least squares discriminant analysis, the exposed to the controlled plant environment and the not exposed groups were clearly separated, and discrimination was due to alteration of fatty acids and alcohols, whose statistical significance was also evaluated. Our results show that NMR-based metabolomics of EBC is a useful tool that has the potential to be successfully used in occupational health to distinguish between subjects exposed and non-exposed to very low airborne levels of chemicals" |
| Keywords: | "Adult Air Pollutants, Occupational/adverse effects/*analysis *Breath Tests Environmental Biomarkers Environmental Monitoring/*methods Humans *Inhalation Exposure/adverse effects Male Masks *Metabolomics *Occupational Exposure/adverse effects *Occupational;" |
| Notes: | "MedlineManiscalco, Mauro Paris, Debora Melck, Dominique Chiariello, Nunzio Di Napoli, Fiorentino Manno, Maurizio Iavicoli, Ivo Motta, Andrea eng Comparative Study Netherlands 2018/10/26 Toxicol Lett. 2018 Dec 1; 298:4-12. doi: 10.1016/j.toxlet.2018.10.018. Epub 2018 Oct 22" |
