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Sci Rep

Title:		Analysis of microbial community structure and volatile compounds in pit mud used for manufacturing Taorong-type Baijiu based on high-throughput sequencing
Author(s):		Liu Y; Sun M; Hou P; Wang W; Shen X; Zhang L; Han S; Pan C;
Address:		"College of Food and Biological Engineering (Liquor College), Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. Postdoctoral Programme, Henan Yangshao Distillery Co., Ltd., Mianchi, 472400, China. School of Life Sciences, Henan University, Kaifeng, 475004, China. Henan Liquor Style Engineering Technology Research Center, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. Zhengzhou Key Laboratory of Liquor Brewing Microbial Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. School of Food and Bio-Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450000, China. Henan Food Industry Science Research Institute Co., Ltd., Zhengzhou, 450003, China. College of Food and Biological Engineering (Liquor College), Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. sige518888@163.com. Henan Liquor Style Engineering Technology Research Center, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. sige518888@163.com. Zhengzhou Key Laboratory of Liquor Brewing Microbial Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China. sige518888@163.com"
Journal Title:		Sci Rep
Year:		2022
Volume:		20220505
Issue:		1
Page Number:		7347 -
DOI:		10.1038/s41598-022-10412-8
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"In this study, the pit mud used in manufacturing Taorong-type Baijiu was collected from the upper, middle, lower and bottom layers of pits at Henan Yangshao Liquor Co., LTD. High-throughput sequencing (HTS) technology was used to analyze the microbial community structure of the pit mud. In addition, the volatile compounds in the pit mud were subjected to preliminary qualitative analysis through headspace-solid phase microextraction and gas chromatography-mass spectrometry (GC-MS). The HTS results demonstrated that there were 5, 3, 5 and 5 dominant bacterial phyla (including 11, 11, 9 and 8 dominant bacterial genera) and 3, 3, 3 and 3 dominant fungal phyla (including 4, 7, 7 and 5 dominant fungal genera) in the pit mud from the F-S (upper), G-Z (middle), H-X (lower) and I-D (bottom) layers, respectively. In the qualitative analysis of the volatile compounds, a total of 77types of volatile compounds were detected in the pit mud, including 46, 45, 39 and 49 types in the pit mud from layers F-S, G-Z, H-X and I-D, respectively. Esters and acids were the two main components of the pit mud. The correlation between the microorganisms present and the main volatile compounds in the pit mud was analyzed. Lentimicrobium, Syner-01 and Blvii28_wastewater-sludge groups were found for the first time in pit mud used for manufacturing Taorong-type Baijiu. The findings of this study could provide a theoretical foundation for improving the quality of pit mud and the flavor of Taorong-type Baijiu"
Keywords:		Alcoholic Beverages/analysis Bacteria/genetics Fermentation High-Throughput Nucleotide Sequencing *Microbiota/genetics *Volatile Organic Compounds/analysis;
Notes:		"MedlineLiu, Yanbo Sun, Mengxiao Hou, Pei Wang, Wenya Shen, Xiangkun Zhang, Lixin Han, Suna Pan, Chunmei eng England 2022/05/06 Sci Rep. 2022 May 5; 12(1):7347. doi: 10.1038/s41598-022-10412-8"