| Title: | Moth desaturase characterized that produces both Z and E isomers of delta 11-tetradecenoic acids |
| Author(s): | Liu W; Jiao H; O'Connor M; Roelofs WL; |
| Address: | "Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA" |
| DOI: | 10.1016/s0965-1748(02)00069-3 |
| ISSN/ISBN: | 0965-1748 (Print) 0965-1748 (Linking) |
| Abstract: | "The redbanded leafroller moth, Argyrotaenia velutinana (Lepidoptera: Tortricidae) uses a 92:8 mixture of (Z)-11- and (E)-11-tetradecenyl acetate in its pheromone blend. These are produced in the abdominal pheromone gland from the corresponding acids, which are biosynthesized in the gland in a 3:2 Z/E ratio by desaturation of myristoyl CoA. The delta 11 desaturase involved in this reaction exhibits unusual substrate and stereospecificities in specifically producing Z11 and E11 isomers of tetradecenoic acid, and exhibiting no activity with C16 and C18 precursor acids. This report describes the cloning and expression of the redbanded leafroller moth delta 11 desaturase, and compares its amino-acid sequence to those of other known insect Z9, Z10, Z11, and E11 desaturases. The metabolic Z9 desaturase from fat body tissue also was cloned and expressed, and found mainly to produce Z9-16:Acid and Z9-18:Acid. The open reading frame of the delta 11 desaturase encodes a protein with 329 amino acids, whereas the open reading frame of the Z9 desaturase encodes a protein with 351 amino acids. Addition of this new delta 11 desaturase with its different substrate and regiospecificites to the databank of characterized integral-membrane desaturases will be key in efforts to determine amino-acid mutations responsible for the wide array of unsaturated fatty-acid products" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence DNA Primers DNA, Complementary/genetics Fatty Acid Desaturases/chemistry/genetics/*metabolism Fatty Acids, Monounsaturated/*metabolism Gene Library Molecular Sequence Data Moths/*enzymology/genetics Open Reading F;" |
| Notes: | "MedlineLiu, Weitian Jiao, Hongmei O'Connor, Marion Roelofs, Wendell L eng Research Support, U.S. Gov't, Non-P.H.S. England 2003/01/18 Insect Biochem Mol Biol. 2002 Nov; 32(11):1489-95. doi: 10.1016/s0965-1748(02)00069-3" |
