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Methods Mol Biol


Title:"Multi-Pronged Investigation of Volatile Compound-Mediated Interactions of Fusarium oxysporum with Plants, Fungi, and Bacteria"
Author(s):Li N; Kang S;
Address:"Department of Plant Pathology & Environmental Microbiology, The Pennsylvania State University, University Park, PA, USA. Department of Plant Pathology & Environmental Microbiology, The Pennsylvania State University, University Park, PA, USA. sxk55@psu.edu"
Journal Title:Methods Mol Biol
Year:2022
Volume:2391
Issue:
Page Number:109 - 127
DOI: 10.1007/978-1-0716-1795-3_10
ISSN/ISBN:1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:"Proteins and many biogenic compounds require water as a medium for movement. However, because volatile compounds (VCs) can travel through the air and porous soils due to their ability to vaporize at ambient temperature, they can mediate diverse intra- and inter-kingdom interactions and perform ecologically functions even in the absence of water. Here, we describe several tools and approaches for investigating how Fusarium oxysporum interacts with plants and other microbes through VCs and how VC-mediated interactions affect its ecology and pathology. We also present a method for capturing F. oxysporum VCs for analysis via gas chromatography linked to mass spectrometry"
Keywords:Bacteria Fungi *Fusarium Gas Chromatography-Mass Spectrometry Plant Diseases Plants Volatile Organic Compounds Water Arabidopsis thaliana Chemical ecology Escherichia coli Fusarium oxysporum Gc-ms I plate Rhizosphere Spme Sandwiched plate assay Trichoderm;
Notes:"MedlineLi, Ningxiao Kang, Seogchan eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2021/10/24 Methods Mol Biol. 2022; 2391:109-127. doi: 10.1007/978-1-0716-1795-3_10"

 
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