| Title: | "Identification of putative odorant binding protein genes in Asecodes hispinarum, a parasitoid of coconut leaf beetle (Brontispa longissima) by antennal RNA-Seq analysis" |
| Author(s): | Li K; Yang X; Xu G; Cao Y; Lu B; Peng Z; |
| Address: | "Institute of Banana and Plantain, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China; Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 570101, China. Electronic address: kemingl@hotmail.com. Texas A&M Agrilife Research and Extension Center, Texas A&M University System, Weslaco, TX 78596, USA. Electronic address: xiangbingyang@yahoo.com. Institute of Banana and Plantain, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China. Electronic address: xuguiying1999@163.com. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address: caoyang110119@126.com. Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 570101, China. Electronic address: lvbaoqian@hotmail.com. Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 570101, China. Electronic address: lypzhq@163.com" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/j.bbrc.2015.10.008 |
| ISSN/ISBN: | 1090-2104 (Electronic) 0006-291X (Linking) |
| Abstract: | "Asecodes hispinarum (Hymenoptera: Eulophidae) is an endoparasitoid and an efficient biological control agent which attacks larvae of Brontispa longissima, a serious insect pest of Palmae plants in China. Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. No previous study has reported on OBPs in A. hispinarum. In this study, we conducted the large-scale identification of OBP genes from the antennae of A. hispinarum by using transcriptome sequencing. Approximately 28.4 million total raw reads and about 27.3 million total clean reads were obtained, and then 46,363 unigenes were assembled. Of these unigenes, a total of 21,263 can be annotated in the NCBI non-redundant database. Among the annotated unigenes, 16,623 of them can be assigned to GO (Gene Ontology). Furthermore, we identified 8 putative OBP genes, and a phylogenetic tree analysis was performed to characterize the 8 OBP genes. In addition, the expression of the 8 OBP genes in different A. hispinarum body tissues was analyzed by real-time quantitative polymerase chain reaction (qRT-PCR). The results indicated that the 8 OBP genes were expressed accordingly to sexes and tissues, but all highly expressed in antennae. The finding of this study will lay the foundation for unraveling molecular mechanisms of A. hispinarum chemoperception" |
| Keywords: | "Amino Acid Sequence Animals Coleoptera/*parasitology Female Hymenoptera/*genetics/*physiology Male Molecular Sequence Data Receptors, Odorant/chemistry/*genetics Sequence Analysis, RNA/*methods Sequence Homology, Amino Acid Transcriptome Asecodes hispinar;" |
| Notes: | "MedlineLi, Keming Yang, Xiangbing Xu, Guiying Cao, Yang Lu, Baoqian Peng, Zhengqiang eng Research Support, Non-U.S. Gov't 2015/10/11 Biochem Biophys Res Commun. 2015 Nov 20; 467(3):514-20. doi: 10.1016/j.bbrc.2015.10.008. Epub 2015 Oct 13" |
