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Int J Biol Macromol

Title:		Involvement of Holotrichia parallela odorant-binding protein 3 in the localization of oviposition sites
Author(s):		Li ET; Wu HJ; Qin JH; Luo J; Li KB; Cao YZ; Zhang S; Peng Y; Yin J;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China; Hubei Key Laboratory of Regional Development and Environmental Response, Faculty of Resources and Environmental Science, Hubei University, Wuhan, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China. Electronic address: kbli@ippcaas.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China. Electronic address: yzcao@ippcaas.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China. Electronic address: szhang@ippcaas.cn. Hubei Key Laboratory of Regional Development and Environmental Response, Faculty of Resources and Environmental Science, Hubei University, Wuhan, China. Electronic address: pengyu@hubu.edu.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, Beijing 100193, China. Electronic address: jyin@ippcaas.cn"
Journal Title:		Int J Biol Macromol
Year:		2023
Volume:		20230505
Issue:		Pt 1
Page Number:		124744 -
DOI:		10.1016/j.ijbiomac.2023.124744
ISSN/ISBN:		1879-0003 (Electronic) 0141-8130 (Linking)
Abstract:		"Organic fertilizers-derived volatiles attract Holotrichia parallela during oviposition. However, the mechanisms underlying the perception of oviposition cues in H. parallela remain unclear. Here, H. parallela odorant-binding protein 3 (HparOBP3) was identified as a key OBP. Bioinformatics analysis showed that HparOBP3 clustered together with Holotrichia oblita OBP8. HparOBP3 was mainly expressed in the antennae of both sexes. Recombinant HparOBP3 exhibited distinct binding affinities towards 22 compounds released by organic fertilizers. After 48 h of RNA interference (RNAi), the expression of HparOBP3 in male and female antennae was decreased by 90.77 % and 82.30 %, respectively. In addition, silencing of HparOBP3 significantly reduced the electrophysiological responses and tropism of males to cis-3-hexen-1-ol, 1-hexanol, and (Z)-beta-ocimene as well as females to cis-3-hexen-1-ol, 1-hexanol, benzaldehyde, and (Z)-beta-ocimene. Molecular docking indicated that hydrophobic residues Leu-83, Leu-87, Phe-108, and Ile-120 of HparOBP3 were important amino acids for interacting with ligands. Mutation of the key residue, Leu-83, significantly diminished the binding ability of HparOBP3. Furthermore, acrylic plastic arena bioassays showed that the attraction and oviposition indexes of organic fertilizers to H. parallela were reduced by 55.78 % and 60.11 %, respectively, after silencing HparOBP3. These results suggest that HparOBP3 is essential in mediating the oviposition behavior of H. parallela"
Keywords:		"Female Male Animals Oviposition Fertilizers Molecular Docking Simulation Insect Proteins/metabolism *Receptors, Odorant/chemistry *Coleoptera/genetics Fluorescence competitive binding assay Holotrichia parallela Molecular docking Odorant binding protein R;"
Notes:		"MedlineLi, Er-Tao Wu, Han-Jia Qin, Jian-Hui Luo, Jing Li, Ke-Bin Cao, Ya-Zhong Zhang, Shuai Peng, Yu Yin, Jiao eng Netherlands 2023/05/07 Int J Biol Macromol. 2023 Jul 1; 242(Pt 1):124744. doi: 10.1016/j.ijbiomac.2023.124744. Epub 2023 May 5"