| Title: | The crystal structure of a cockroach pheromone-binding protein suggests a new ligand binding and release mechanism |
| Author(s): | Lartigue A; Gruez A; Spinelli S; Riviere S; Brossut R; Tegoni M; Cambillau C; |
| Address: | "Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Universites Aix-Marseille I & 2, 31 Ch. Joseph Aiguier, 13402 Marseille Cedex 20, France" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "Pheromone-binding proteins (PBPs) are small helical proteins found in sensorial organs, particularly in the antennae, of moth and other insect species. They were proposed to solubilize and carry the hydrophobic pheromonal compounds through the antennal lymph to receptors, participating thus in the peri-receptor events of signal transduction. The x-ray structure of Bombyx mori PBP (BmorPBP), from male antennae, revealed a six-helix fold forming a cavity that contains the pheromone bombykol. We have identified a PBP (LmaPBP) from the cockroach Leucophaea maderae in the antennae of the females, the gender attracted by pheromones in this species. Here we report the crystal structure of LmaPBP alone or in complex with a fluorescent reporter (amino-naphthalen sulfonate, ANS) or with a component of the pheromonal blend, 3-hydroxy-butan-2-one. Both compounds bind in the internal cavity of LmaPBP, which is more hydrophilic than BmorPBP cavity. LmaPBP structure ends just after the sixth helix (helix F). BmorPBP structure extends beyond the sixth helix with a stretch of residues elongated at neutral pH and folding as a seventh internalized helix at low pH. These differences between LmaPBP and BmorPBP structures suggest that different binding and release mechanism may be adapted to the hydrophilicity or hydrophobicity of the pheromonal ligand" |
| Keywords: | "Amino Acid Sequence Animals Bombyx Butanones/pharmacology Carrier Proteins/*chemistry Cloning, Molecular Cockroaches Crystallography, X-Ray Female Fluorescent Dyes/pharmacology Hydrogen-Ion Concentration *Insect Proteins Kinetics Ligands Models, Molecular;" |
| Notes: | "MedlineLartigue, Audrey Gruez, Arnaud Spinelli, Silvia Riviere, Stephane Brossut, Remy Tegoni, Mariella Cambillau, Christian eng Research Support, Non-U.S. Gov't 2003/05/27 J Biol Chem. 2003 Aug 8; 278(32):30213-8. doi: 10.1074/jbc.M304688200. Epub 2003 May 23" |
