Title: | MALDI MSI and Raman Spectroscopy Application in the Analysis of the Structural Components and Flavonoids in Brassica napus Stem |
Author(s): | Krysa M; Susniak K; Kubas A; Kidaj D; Sroka-Bartnicka A; |
Address: | "Independent Unit of Spectroscopy and Chemical Imaging, Faculty of Biomedicine, Medical University of Lublin, Chodzki 4a Street, 20-093 Lublin, Poland. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, Chodzki Street 1, 20-093 Lublin, Poland. Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland" |
ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
Abstract: | "Nod factors among the signaling molecules produced by rhizobia in response to flavonoids to induce root nodule formation in the legumes. It is, however, hypothesized that they might increase the yield and positively impact the growth of non-legumes. To evaluate this statement, rapeseed treated with Nod factor-based biofertilizers were cultivated, their stems was collected, and the metabolic changes were investigated using Raman spectroscopy and MALDI mass spectrometry imaging. Biofertilizer proved to increase the concentration of lignin in the cortex, as well as hemicellulose, pectin, and cellulose in the pith. Moreover, the concentration of quercetin derivatives and kaempferol derivatives increased, while the concentration of isorhamnetin dihexoside decreased. The increase in the concentration of the structural components in the stem might therefore increase the lodging resistance, while the increase in concentration of the flavonoids might increase their resistance to fungal infection and herbivorous insects" |
Keywords: | Brassica napus MALDI mass spectrometry imaging Nod factors Raman spectroscopy biofertilizer rapeseed; |
Notes: | "PubMed-not-MEDLINEKrysa, Mikolaj Susniak, Katarzyna Kubas, Adrianna Kidaj, Dominika Sroka-Bartnicka, Anna eng LIDER/11/0070/L-8/16/NCBR/2017/National Centre for Research and Development/ Switzerland 2023/06/27 Metabolites. 2023 May 25; 13(6):687. doi: 10.3390/metabo13060687" |