| Title: | Females with impaired ovarian function could be vulnerable to environmental pollutants: identification via next-generation sequencing of the vaginal microbiome |
| Author(s): | Kim S; Lee SH; Min KJ; Lee S; Hong JH; Song JY; Lee JK; Lee NW; Lee E; |
| Address: | "Department of Obstetrics and Gynecology, CHA Ilsan Medical Center, CHA University School of Medicine, Goyang-si, Republic of Korea. Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea. Department of Medical Science, Korea University Graduate School, Seoul, Republic of Korea. Department of Preventive Medicine, Korea University College of Medicine, Seoul, Republic of Korea" |
| DOI: | 10.1080/01443615.2021.2006162 |
| ISSN/ISBN: | 1364-6893 (Electronic) 0144-3615 (Linking) |
| Abstract: | "The vaginal microbiome has been widely investigated. However, its relationship with impaired ovarian function has not been evaluated. We conducted a next-generation sequencing (NGS) study of the vaginal microbiome in females with normal and decreased ovarian function and analysed its sensitivity to environmental pollutants. Vaginal swabs were collected from 92 individuals (22 with impaired ovarian function). The 16S rDNA sequences were assembled by FLASH and clustered in OTUs. Diversity analysis was performed using QIIME. The impaired function group showed lower AMH (p < .01) and higher FSH (p = .04). Only two species showed significant differences: Propionibacterium acnes and Prevotella copri. Moreover, more environmental pollutants were related to changes in the vaginal microbiome in the impaired ovarian function group than in the normal group. Vaginal microbiomes in young women with decreased ovarian function tended to be more sensitive to environmental pollutants, especially volatile organic compounds.Impact StatementWhat is already known on this subject? In this study, the possible influence of environmental pollutants, especially volatile organic compounds to ovarian function were identified via next-generation sequencing.What do the results of this study add? This is the first study that shows vaginal microbiomes in young women with decreased ovarian function to be more sensitive to environmental pollutants.What are the implications of these findings for clinical practice and/or further research? The association between impaired ovarian function and environmental pollutants from this study could be helpful when counselling patients with POI" |
| Keywords: | "*Environmental Pollutants/adverse effects Female High-Throughput Nucleotide Sequencing Humans *Microbiota/genetics RNA, Ribosomal, 16S/genetics Vagina/microbiology *Volatile Organic Compounds Vaginal microbiome environmental pollutants next-generation seq;" |
| Notes: | "MedlineKim, Seongmin Lee, Se Hee Min, Kyung Jin Lee, Sanghoon Hong, Jin Hwa Song, Jae Yun Lee, Jae Kwan Lee, Nak Woo Lee, Eunil eng England 2022/01/09 J Obstet Gynaecol. 2022 Jul; 42(5):1482-1488. doi: 10.1080/01443615.2021.2006162. Epub 2022 Jan 7" |
