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PLoS One

Title:		"Acetone application for administration of bioactive substances has no negative effects on longevity, fitness, and sexual communication in a parasitic wasp"
Author(s):		Jatsch AS; Ruther J;
Address:		"Institute for Zoology, University of Regensburg, Regensburg, Germany"
Journal Title:		PLoS One
Year:		2021
Volume:		20210120
Issue:		1
Page Number:		e0245698 -
DOI:		10.1371/journal.pone.0245698
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Administration of defined amounts of bioactive substances is a perseverative problem in physiological studies on insects. Apart from feeding and injection, topical application of solutions of the chemicals is most commonly used for this purpose. The solvents used should be non-toxic and have least possible effects on the studied parameters. Acetone is widely used for administration of chemical substances to insects, but possible side-effects of acetone application on fitness and behavioral parameters have been rarely investigated. Here we study the effects of acetone application (207 nl) on fitness and sexual communication in the parasitic wasp Nasonia giraulti Darling. Application of acetone had neither negative effects on longevity nor on offspring number and offspring sex ratio of treated wasps. Treatment of females hampered courtship and mating of N. giraulti couples neither directly after application nor one day after. Male sex pheromone titers were not influenced by acetone treatment. Three application examples demonstrate that topical acetone application is capable of bringing active amounts of insect hormones, neuromodulators, and biosynthetic precursors even in tiny insects. We advocate the use of acetone as a convenient, conservative, and broadly applicable vehicle for studying the effects of bioactive substances in insects"
Keywords:		"Acetone/*pharmacology Animals Female Longevity/*drug effects Male Sex Attractants/metabolism/*pharmacology Sexual Behavior, Animal/*drug effects Wasps/*physiology;"
Notes:		"MedlineJatsch, Anne-Sophie Ruther, Joachim eng 2021/01/21 PLoS One. 2021 Jan 20; 16(1):e0245698. doi: 10.1371/journal.pone.0245698. eCollection 2021"