« Previous Abstract"OH production from the photolysis of isoprene-derived peroxy radicals: cross-sections, quantum yields and atmospheric implications"    Next Abstract"Odorant-odorant metabolic interaction, a novel actor in olfactory perception and behavioral responsiveness" »

Cell

Title:		In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor
Author(s):		Hansen W; Garcia PD; Walter P;
Address:
Journal Title:		Cell
Year:		1986
Volume:		45
Issue:		3
Page Number:		397 - 406
DOI:		10.1016/0092-8674(86)90325-9
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"The in vitro synthesized precursor of the alpha-factor pheromone, prepro-alpha-factor, of Saccharomyces cerevisiae was translocated across yeast microsomal membranes in either a homologous or a wheat germ cell free system. Translocated prepro-alpha-factor was glycosylated, sedimented with yeast microsomal vesicles, and was protected from digestion by added protease, but was soluble after alkaline sodium carbonate treatment. Thus prepro-alpha-factor was properly sequestered within yeast microsomal vesicles, but was not integrated into the lipid bilayer. In marked contrast to protein translocation across mammalian microsomal membranes, translocation of prepro-alpha-factor across yeast microsomal membranes could occur posttranslationally. This reaction required protein components in the yeast microsomal fraction that could be inactivated by alkylation or proteolysis, was ATP-dependent, and was insensitive to the presence of a variety of uncouplers and ionophores"
Keywords:		"Adenosine Triphosphate/metabolism Animals Biological Transport Cell-Free System Dogs Endoplasmic Reticulum/*metabolism Fungal Proteins/*metabolism Mating Factor Peptides/*metabolism *Protein Processing, Post-Translational Ribonucleoproteins/metabolism Sac;"
Notes:		"MedlineHansen, W Garcia, P D Walter, P eng GM-10562/GM/NIGMS NIH HHS/ GM-32384/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1986/05/09 Cell. 1986 May 9; 45(3):397-406. doi: 10.1016/0092-8674(86)90325-9"