| Title: | Application of Volatile Organic Compound Analysis in a Nutritional Intervention Study: Differential Responses during Five Hours Following Consumption of a High- and a Low-Fat Dairy Drink |
| Author(s): | Hageman JHJ; Nieuwenhuizen AG; van Ruth SM; Hageman JA; Keijer J; |
| Address: | "Human and Animal Physiology, Wageningen University, 6708 WD, Wageningen, The Netherlands. FrieslandCampina, 3818 LE, Amersfoort, The Netherlands. Food Quality and Design/RIKILT, Wageningen University and Research, 6700 AE, Wageningen, The Netherlands. Biometris, Wageningen University and Research, 6700 AA, Wageningen, The Netherlands" |
| ISSN/ISBN: | 1613-4133 (Electronic) 1613-4125 (Print) 1613-4125 (Linking) |
| Abstract: | "SCOPE: Exhaled volatile organic compounds (VOCs) are a possible relevant target for noninvasive assessment of metabolic responses. Using a breathomics approach, it is aimed to explore whether lipid intake influences VOC profiles in exhaled air, and to obtain insight in intra- and interindividual variations. METHODS AND RESULTS: Three human interventions are performed. In the first, 12 males consume a high-fat drink on three study days. In the second, 12 males receive a high- and a low-fat drink on 6 days. In the third, three volunteers consume the high-fat drink again for tentative compound identification. Participants are asked to exhale, for 5 h postprandial with 15-20 min intervals, into a proton-transfer-reaction mass spectrometer, and VOCs in exhaled air are measured. Consumption of a drink alters the VOC profile, with considerable interindividual variation and quantitative intraindividual differences between days. Consumption of two different drinks results in a distinct VOC profile, caused by several specific m/z values. Most of these compounds are identified as being related to ketone body formation and lipid oxidation, showing an increase in high- versus low-fat drink. CONCLUSION: Exhaled VOCs have the potential to assess differences in metabolic responses induced by nutrition, especially when day-to-day variation can be minimized" |
| Keywords: | Adult Breath Tests Dairy Products Dietary Fats/*administration & dosage Drinking Humans Male Mass Spectrometry *Nutrition Assessment Time Factors Volatile Organic Compounds/*analysis breath analysis breathomics inter- and intraindividual variation lipids; |
| Notes: | "MedlineHageman, Jeske H J Nieuwenhuizen, Arie G van Ruth, Saskia M Hageman, Jos A Keijer, Jaap eng Research Support, Non-U.S. Gov't Germany 2019/07/22 Mol Nutr Food Res. 2019 Oct; 63(20):e1900189. doi: 10.1002/mnfr.201900189. Epub 2019 Aug 5" |
