| Title: | Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica |
| Author(s): | Freitas CSA; Maciel LF; Correa Dos Santos RA; Costa O; Maia FCB; Rabelo RS; Franco HCJ; Alves E; Consonni SR; Freitas RO; Persinoti GF; Oliveira JVC; |
| Address: | "Brazilian Biorenewable National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo, Brazil. Genetics and Molecular Biology Graduate Program, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil. School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo, Brazil. Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo, Brazil. Cropman Agricultural Innovation, Campinas, Sao Paulo, Brazil. Laboratory of Electron Microscopy and Ultrastructural Analysis, Plant Pathology Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil. Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "Due to an increasing demand for sustainable agricultural practices, the adoption of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged as an eco-friendly alternative to the use of agrochemicals. Here, we identified three Pseudomonas strains that were able to inhibit, in vitro, up to 80% of mycelial growth of the phytopathogenic fungus Thielaviopsis ethacetica, the causal agent of pineapple sett rot disease in sugarcane. Using GC/MS, we found that these bacteria produced 62 different VOCs, and further functional validation revealed compounds with high antagonistic activity to T. ethacetica. Transcriptomic analysis of the fungal response to VOCs indicated that these metabolites downregulated genes related to fungal central metabolism, such as those involved in carbohydrate metabolism. Interestingly, genes related to the DNA damage response were upregulated, and micro-FTIR analysis corroborated our hypothesis that VOCs triggered DNA damage. Electron microscopy analysis showed critical morphological changes in mycelia treated with VOCs. Altogether, these results indicated that VOCs hampered fungal growth and could lead to cell death. This study represents the first demonstration of the molecular mechanisms involved in the antagonism of sugarcane phytopathogens by VOCs and reinforces that VOCs can be a sustainable alternative for use in phytopathogen biocontrol" |
| Keywords: | *Ascomycota/metabolism Bacteria/metabolism DNA Damage *Saccharum *Volatile Organic Compounds/metabolism/pharmacology; |
| Notes: | "MedlineFreitas, Carla Sant Anna Maciel, Lucas Ferreira Correa Dos Santos, Renato Augusto Costa, Ohanna Maria Menezes Madeiro Maia, Francisco Carlos Barbosa Rabelo, Renata Santos Franco, Henrique Coutinho Junqueira Alves, Eduardo Consonni, Silvio Roberto Freitas, Raul Oliveira Persinoti, Gabriela Felix Oliveira, Juliana Velasco de Castro eng Research Support, Non-U.S. Gov't England 2022/01/08 Environ Microbiol. 2022 Mar; 24(3):1430-1453. doi: 10.1111/1462-2920.15876. Epub 2022 Jan 7" |
