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J Sep Sci

Title:		Group-type quantitative analysis of flavor compounds in ripening avocados
Author(s):		Ferranti A; Hayward T;
Address:		"Analytical Sciences, Apeel Sciences, 71 S Los Carneros Rd, Goleta, CA, 93117, USA"
Journal Title:		J Sep Sci
Year:		2022
Volume:		20220315
Issue:		10
Page Number:		1737 - 1745
DOI:		10.1002/jssc.202100577
ISSN/ISBN:		1615-9314 (Electronic) 1615-9306 (Linking)
Abstract:		"Avocados are a superfood gaining popularity in people's diet. Profiling and quantifying the volatiles associated with flavor can further help in understanding the fruit. However, this is challenging due to relatively low abundance of volatile compounds. The complex mixtures inherent to avocado flavor can result in coelutions using classical chromatographic techniques. To overcome these challenges, solid-phase microextraction was used to extract and preconcentrate volatiles, then separated and quantified using two-dimensional gas chromatography with a flame ionization detector. This technique enhances separation power and produces well-ordered chromatograms, allowing for templated groupings of compounds of similar chemical composition into regions. Using the flame ionization detector, an average response factor was determined and used for quantification of these templated group-type regions, as well as individual compounds. This group-type quantification improved the overall precision of compound classes in 50 avocados by at least a factor of 2, when compared to that of the individual components. Overall, the abundance of associated flavor groups, such as terpenes and alcohols decreased, whereas aldehyde groups remained constant throughout ripening. The combination of solid-phase microextraction with two-dimensional gas chromatography and group-type quantification allows for an overall better understanding of the volatiles associated with flavor of avocados"
Keywords:		Flame Ionization Gas Chromatography-Mass Spectrometry/methods Humans *Persea Solid Phase Microextraction/methods *Volatile Organic Compounds/analysis Avocado comprehensive two-dimensional gas chromatography flavor profiling solid phase microextraction vol;
Notes:		"MedlineFerranti, Allison Hayward, Taylor eng Germany 2022/03/06 J Sep Sci. 2022 May; 45(10):1737-1745. doi: 10.1002/jssc.202100577. Epub 2022 Mar 15"