| Title: | Molecular characterization and functional differentiation of three pheromone-binding proteins from Tryporyza intacta |
| Author(s): | Fang N; Hu Y; Mao B; Bi J; Zheng Y; Guan C; Wang Y; Li J; Mao Y; Ai H; |
| Address: | "Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China. Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou, 510316, China. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China. aihui@mail.ccnu.edu.cn" |
| DOI: | 10.1038/s41598-018-29164-5 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Insect pheromone-binding proteins (PBPs) have been proposed to capture and transport hydrophobic sex pheromone components emitted by con-specific insects to pheromone receptors in the hemolymph of male antennal sensilla. In this study, field trapping results indicate that a mixture of E11-16: Ald and Z11-16: Ald can effectively attract a great number of male Tryporyza intacta. Real-time PCR results suggest that the transcript levels of three TintPBP1-3 genes are mainly expressed in the adult antennae. Fluorescence competitive binding experiments show that TintPBP1-3 proteins have great binding affinities to their major sex pheromones. Moreover, TintPBPs clearly cannot bind to other four kinds of sex pheromone components released by another sugarcane borer, Chilo venosatus and Chilo infuscatellu, which have the same host plant and live in similar habitats like T. intacta. The molecular docking results demonstrate that six amino acid residues of the three TintPBPs are crucial for the specific perception of the sex pheromone components. These results will provide a foundation for the development of novel sex pheromone analogues and blocking agents for biological control of sugarcane pests, improving their efficient monitoring and integrated management strategies in the sugarcane field" |
| Keywords: | "Animals Female Gene Expression Profiling Insect Proteins/*chemistry/physiology Male Molecular Docking Simulation Moths/genetics/*metabolism Phylogeny Receptors, Pheromone Sequence Alignment Sequence Analysis, Protein Sex Attractants/*chemistry/physiology;" |
| Notes: | "MedlineFang, Nainai Hu, Yuwei Mao, Bin Bi, Jie Zheng, Ya Guan, Chuxiong Wang, Yufeng Li, Jihu Mao, Yongkai Ai, Hui eng 31472052/National Natural Science Foundation of China (National Science Foundation of China)/International Research Support, Non-U.S. Gov't England 2018/07/19 Sci Rep. 2018 Jul 17; 8(1):10774. doi: 10.1038/s41598-018-29164-5" |
