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Metabolomics

Title:		Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity
Author(s):		Eckert S; Eilers EJ; Jakobs R; Anaia RA; Aragam KS; Bloss T; Popp M; Sasidharan R; Schnitzler JP; Stein F; Steppuhn A; Unsicker SB; van Dam NM; Yepes S; Ziaja D; Muller C;
Address:		"Department of Chemical Ecology, Bielefeld University, Bielefeld, Germany. Institute of Biodiversity, Friedrich Schiller University Jena, Jena, Germany. Molecular Interaction Ecology, German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany. Department of Molecular Botany, Hohenheim University, Stuttgart, Germany. Research Unit Environmental Simulation, Helmholtz Zentrum Munchen, Munich, Germany. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Jena, Germany. Leibniz Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany. Department of Chemical Ecology, Bielefeld University, Bielefeld, Germany. caroline.mueller@uni-bielefeld.de"
Journal Title:		Metabolomics
Year:		2023
Volume:		20230623
Issue:		7
Page Number:		62 -
DOI:		10.1007/s11306-023-02026-6
ISSN/ISBN:		1573-3890 (Electronic) 1573-3882 (Print) 1573-3882 (Linking)
Abstract:		"INTRODUCTION: Assessing intraspecific variation in plant volatile organic compounds (VOCs) involves pitfalls that may bias biological interpretation, particularly when several laboratories collaborate on joint projects. Comparative, inter-laboratory ring trials can inform on the reproducibility of such analyses. OBJECTIVES: In a ring trial involving five laboratories, we investigated the reproducibility of VOC collections with polydimethylsiloxane (PDMS) and analyses by thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). As model plant we used Tanacetum vulgare, which shows a remarkable diversity in terpenoids, forming so-called chemotypes. We performed our ring-trial with two chemotypes to examine the sources of technical variation in plant VOC measurements during pre-analytical, analytical, and post-analytical steps. METHODS: Monoclonal root cuttings were generated in one laboratory and distributed to five laboratories, in which plants were grown under laboratory-specific conditions. VOCs were collected on PDMS tubes from all plants before and after a jasmonic acid (JA) treatment. Thereafter, each laboratory (donors) sent a subset of tubes to four of the other laboratories (recipients), which performed TD-GC-MS with their own established procedures. RESULTS: Chemotype-specific differences in VOC profiles were detected but with an overall high variation both across donor and recipient laboratories. JA-induced changes in VOC profiles were not reproducible. Laboratory-specific growth conditions led to phenotypic variation that affected the resulting VOC profiles. CONCLUSION: Our ring trial shows that despite large efforts to standardise each VOC measurement step, the outcomes differed both qualitatively and quantitatively. Our results reveal sources of variation in plant VOC research and may help to avoid systematic errors in similar experiments"
Keywords:		*Volatile Organic Compounds/analysis Reproducibility of Results Metabolomics Terpenes/analysis Gas Chromatography-Mass Spectrometry/methods Plants Plant metabolites Proficiency test Quality assurance Quality control Reproducibility Standardisation Static;
Notes:		"MedlineEckert, Silvia Eilers, Elisabeth J Jakobs, Ruth Anaia, Redouan Adam Aragam, Kruthika Sen Bloss, Tanja Popp, Moritz Sasidharan, Rohit Schnitzler, Jorg-Peter Stein, Florian Steppuhn, Anke Unsicker, Sybille B van Dam, Nicole M Yepes, Sol Ziaja, Dominik Muller, Caroline eng MU1829/29-1/Deutsche Forschungsgemeinschaft/ 2023/06/23 Metabolomics. 2023 Jun 23; 19(7):62. doi: 10.1007/s11306-023-02026-6"