| Title: | High killing rate of nematode and promotion of rice growth by synthetic volatiles from Bacillus strains due to enhanced oxidative stress response |
| Author(s): | Ali Q; Yu C; Wang Y; Sheng T; Zhao X; Wu X; Jing L; Gu Q; Wu H; Gao X; |
| Address: | "Key Laboratory of Integrated Management of Crop Diseases and Pests, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China" |
| ISSN/ISBN: | 1399-3054 (Electronic) 0031-9317 (Linking) |
| Abstract: | "The plant parasitic nematode Aphelenchoides besseyi is a major pest that poses serious threats to different vegetables and crop plants. In the present study, volatiles isolated from Bacillus spp. were utilized as green biocontrol agents to overcome nematodes. In in vitro experiment, Bacillus spp. GBSC56, SYST2, and FZB42 showed the strongest nematicidal activity with killing rates of 80.78%, 75.69%, and 60.45%, respectively, as compared with control. The selected synthetic volatile organic compounds (VOCs), namely albuterol, benzaldehyde (BDH), 1,2-benzisothiazol-3(2H)-one (1,2-HIT), dimethyl disulfide (DMDS), 2-undecanone (2-UD), and 1,3-propanediole (1,3-PD), exhibited strong nematicidal activity, with A. besseyi killing rate of 85.58%, 82.65%, 81.75%, 80.36%, 84.45%, and 82.36%, respectively, at 400 mug/mL. Microscopic analysis proved that the rapid mortality was due to the production of reactive oxygen species (ROS). Molecular docking attributed this ROS production to the nematicidal effect of synthetic VOCs on NADH DEHYDROGENASE SUBUNIT 2, which is known to play a critical role in the suppression of ROS in nematode models. In a greenhouse experiment, the Bacillus strains GBSC56, SYST2, and FZB42 and their synthetic VOCs significantly improved the physiological parameters in terms of growth promotion traits. In addition, selected genes related to growth promotion and defense genes showed a significant upregulation of their expression in rice seedlings treated with those synthetic VOCs. Overall, these findings revealed that the selected Bacillus strains and their synthetic VOCs possess high potential against A. besseyi. Moreover, this study also sheds new light on the mechanisms by which specific Bacillus nematicidal VOCs influence important genes involved in rice plant growth promotion and could effectively be used to suppress plant parasitic nematodes" |
| Keywords: | Animals *Bacillus *Oryza Reactive Oxygen Species/metabolism Molecular Docking Simulation *Nematoda Plants Oxidative Stress; |
| Notes: | "MedlineAli, Qurban Yu, Chenjie Wang, Yujie Sheng, Tao Zhao, Xiaozhen Wu, Xiaohui Jing, Liang Gu, Qin Wu, Huijun Gao, Xuewen eng U20A2039/The Key Project of NSFC Regional Innovation and Development Joint Fund/ 2022YFE0121800/National Key R&D Plan Intergovernmental International Science and Technology Innovation Cooperation Project/ BK20200078/the Natural Science Foundation for Excellent Youth Scholars of Jiangsu Province, China/ 2021RXTDLX10/the Ningxia Hui Autonomous Region Introduction Team Project/ NAUSY-M18/the Guidance Foundation of the Sanya Institute of Nanjing Agricultural University/ KYZZ2022001/Fundamental Research Funds for the Central Universities/ Denmark 2023/02/02 Physiol Plant. 2023 Jan; 175(1):e13868. doi: 10.1111/ppl.13868" |
