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Mol Biol Cell

Title:		The adhesin Hwp1 and the first daughter cell localize to the a/a portion of the conjugation bridge during Candida albicans mating
Author(s):		Daniels KJ; Lockhart SR; Staab JF; Sundstrom P; Soll DR;
Address:		"Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242-1324, USA"
Journal Title:		Mol Biol Cell
Year:		2003
Volume:		20031017
Issue:		12
Page Number:		4920 - 4930
DOI:		10.1091/mbc.e03-04-0264
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"The cell wall protein Hwp1 was originally demonstrated to be expressed exclusively in hyphae of Candida albicans and cross-linked to human epithelium by mammalian transglutaminase. Hwp1 is expressed on the walls of hyphae formed by a/alpha, a/a, and alpha/alpha cells. Hence, it is expressed on hyphae independently of mating type. However, Hwp1 is selectively expressed on the wall of conjugation tubes formed by a/a cells, but not alpha/alpha cells, in the mating process. This was demonstrated in all possible crosses between four unrelated natural a/a strains and four unrelated alpha/alpha strains. In zygotes, Hwp1 is restricted to that portion of the wall of the conjugation bridge contributed by the a/a parent cell. Hwp1 staining further revealed that the first daughter bud that emerges from the conjugation bridge does so from the a/a-contributed portion. Hwp1 expression and localization during the mating process is, therefore, mating type specific, opaque phase specific, and alpha-pheromone induced. These results indicate that the mating type-specific contributions to the conjugation bridge during the mating process in C. albicans are qualitatively and functionally distinct and that the a/a portion of the bridge, which selectively contains Hwp1, bears the first daughter cell in the mating process"
Keywords:		"Blotting, Northern Candida albicans/cytology/genetics/*metabolism Cell Adhesion/physiology Cell Wall/genetics/metabolism Fluorescent Antibody Technique Fungal Proteins/genetics/*metabolism Genes, Fungal/genetics Genes, Mating Type, Fungal Hyphae/cytology/;"
Notes:		"MedlineDaniels, Karla J Lockhart, Shawn R Staab, Janet F Sundstrom, Paula Soll, David R eng R01 DE011375/DE/NIDCR NIH HHS/ AI2392/AI/NIAID NIH HHS/ DE11375/DE/NIDCR NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2003/10/21 Mol Biol Cell. 2003 Dec; 14(12):4920-30. doi: 10.1091/mbc.e03-04-0264. Epub 2003 Oct 17"