| Title: | An improved microtitre enzyme immunoassay to measure the boar taint steroid 5alpha-androst-16-en-3-one in blood plasma of pigs |
| Author(s): | Claus R; Lacorn M; Ostertag C; |
| Address: | "Institute of Animal Husbandry and Animal Breeding, Section of Animal Husbandry and Regulation Physiology, University of Hohenheim, Garbenstr. 17, 70599 Stuttgart, Germany" |
| DOI: | 10.1016/j.meatsci.2008.04.019 |
| ISSN/ISBN: | 0309-1740 (Print) 0309-1740 (Linking) |
| Abstract: | "Androstenone (5alpha-androst-16-en-3-one) is a volatile steroid which is synthesized in boar testes and stored in high amounts in fat thus leading to an urine-like odor in pork. Whereas microtiter assays (MTA) exist for fat determination, measurement in blood with MTA was not yet possible. The system reported here is based on a specific antiserum and a horse radish peroxidase conjugate as tracer. For coating bovine serum albumin was avoided which otherwise would lead to unspecific binding. Blood plasma was extracted with n-hexane, solvent was evaporated in a vacuum centrifuge and the extract was taken up in buffer with 10% methanol. Assay sensitivity was 2.5pg/well (0.025ng/mL), specificity was confirmed by GC-MS (r=0.96; n=50) and inter- and intraassay variation were 4.9% and 5.9%, respectively. Thus an assay system is available for studies in pig production and in wildlife research due to pheromone activity also in other species" |
| Notes: | "PubMed-not-MEDLINEClaus, Rolf Lacorn, Markus Ostertag, Carmen eng England 2008/11/01 Meat Sci. 2008 Nov; 80(3):934-8. doi: 10.1016/j.meatsci.2008.04.019. Epub 2008 Apr 29" |
