| Title: | "Oviposition Deterrents in Larval Frass of Potato Tuberworm Moth, Phthorimaea operculella (Lepidoptera: Gelechiidae)" |
| Author(s): | Zhang XG; Li X; Gao YL; Liu Y; Dong WX; Xiao C; |
| Address: | "State Key Lab for Conservation and Utilization of Biological Resources in Yunnan, College of Plant Protection, Yunnan Agricultural Univ, Kunming, 650201, China. State Key Lab for Biology of Plant Diseases and Insect Pests, Institute of Plant protection, Chinese Academy of Agricultural Sciences, Beijing, China. State Key Lab for Conservation and Utilization of Biological Resources in Yunnan, College of Plant Protection, Yunnan Agricultural Univ, Kunming, 650201, China. dongwenxia@163.com" |
| DOI: | 10.1007/s13744-018-0655-y |
| ISSN/ISBN: | 1678-8052 (Electronic) 1519-566X (Linking) |
| Abstract: | "The potato tuberworm moth (PTM) Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) is one of the most damaging pests of potato Solanum tuberosum L. in warm temperate and subtropical areas. Our previous experiment showed that extracts of larval frass of PTM deterred oviposition of conspecific females. In this study, we investigated the identification of chemicals in larval frass that were influencing the oviposition of PTM by behavioral bioassays and electroantennography analysis in the laboratory. Frass was collected from third and fourth instar larvae and combined analysis of gas chromatography coupled with electroantennography (GC-EAD) of dichloromethane extracts showed that eight compounds from larval frass extracts elicited repeatable antennal responses from mated females. Seven EAD-active compounds in frass volatile extract were identified by gas chromatography-mass spectrometry (GC-MS) as linoleic acid, octadecanoic acid, tricosane, pentacosane, heptacosane, nonacosane, and cholesterol. Oviposition bioassays indicated that frass extracts had a deterrent effect on egg laying, the deterrent activity increased with the concentration of frass extracts, and the threshold value for statistical significance in oviposition deterrence was in the range of 20-200 mg frass per cage. Linoleic acid, pentacosane, heptacosane, nonacosane, and cholesterol in larval frass volatiles were found to play a key role in repelling oviposition in a dose-dependent manner. We suggest that the bioactive compounds in larval frass are responsible for repelling oviposition of PTM, and n-alkanes, especially pentacosane, strongly deter oviposition and may be considered as a potential oviposition deterrent for potential applications" |
| Keywords: | Animals Feces/*chemistry Female Gas Chromatography-Mass Spectrometry Insect Repellents Larva/*chemistry Moths/*chemistry *Oviposition Solanum tuberosum Volatile Organic Compounds/*chemistry Frass volatile extracts conspecific females oviposition bioassay; |
| Notes: | "MedlineZhang, X G Li, X Gao, Y L Liu, Y Dong, W X Xiao, C eng 31560607/National Natural Science Foundation of China/ Netherlands 2018/12/13 Neotrop Entomol. 2019 Jun; 48(3):496-502. doi: 10.1007/s13744-018-0655-y. Epub 2018 Dec 11" |
