| Title: | Transcriptomic profiling of the floral fragrance biosynthesis pathway of Liriodendron and functional characterization of the LtuDXR gene |
| Author(s): | Zhang C; Liu H; Hu S; Zong Y; Xia H; Li H; |
| Address: | "Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. Electronic address: hgli@njfu.edu.cn" |
| DOI: | 10.1016/j.plantsci.2021.111124 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "Floral fragrance, which has the function of attracting pollinators, is a class of volatile secondary metabolites mainly released by the secretory tissue of petals. Terpenoids are key components of floral volatile substances. Previous studies have shown that there are significant differences in the concentration and composition of volatile floral fragrances, especially terpenoids, between Liriodendron chinense and L. tulipifera. At present, the mechanism by which the synthesis of floral fragrance is regulated in Liriodendron remains unexplored. In this study, we analyzed the differentially expressed genes (DEGs) of L. chinense and L. tulipifera, and identified 130 DEGs related to terpenoid synthesis. A KEGG enrichment analysis of DEGs related to terpenoid biosynthesis revealed that the monoterpenoid biosynthesis pathway was the most significant. We cloned the LtuDXR gene from L. tulipifera using RACE technology. RT-qPCR results showed that the expression of the LtuDXR gene was the highest in the early florescence petals, indicating that the LtuDXR gene may play a role in the synthesis of volatile terpenoids. Subcellular localization showed that the LtuDXR protein is mainly localized in the chloroplast. Overexpression of LtuDXR in Arabidopsis thaliana significantly increased the plant height, DXR enzyme activity, and carotenoid content. In this study, we identified and functionally characterized LtuDXR, which is involved in terpenoid synthesis in Liriodendron. Our work lays the foundation for further exploration of the molecular mechanism by which terpenoid biosynthesis is regulated in Liriodendron" |
| Keywords: | "Biosynthetic Pathways/*genetics Flowers/*genetics/*metabolism Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Genetic Variation Genotype Liriodendron/*genetics/*metabolism *Odorants Terpenes/*metabolism 1-deoxy-d-xylulose-5-phosph;" |
| Notes: | "MedlineZhang, Chengge Liu, Huanhuan Hu, Shan Zong, Yaxian Xia, Hui Li, Huogen eng Comparative Study Ireland 2021/12/14 Plant Sci. 2022 Jan; 314:111124. doi: 10.1016/j.plantsci.2021.111124. Epub 2021 Nov 20" |
