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Anal Bioanal Chem

Title:		"Simultaneous determination of bioactive constituents in Danggui Buxue Tang for quality control by HPLC coupled with a diode array detector, an evaporative light scattering detector and mass spectrometry"
Author(s):		Yi L; Qi LW; Li P; Ma YH; Luo YJ; Li HY;
Address:		"Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China"
Journal Title:		Anal Bioanal Chem
Year:		2007
Volume:		20070725
Issue:		2
Page Number:		571 - 580
DOI:		10.1007/s00216-007-1431-8
ISSN/ISBN:		1618-2642 (Print) 1618-2642 (Linking)
Abstract:		"Danggui Buxue Tang (DBT), a classical traditional Chinese formula comprising Radix Angelicae Sinensis (RAS) and Radix Astragali (RA), has been widely used to treat menopausal irregularity in Chinese women for nearly 800 years. In this study, a comprehensive analytical method of simultaneously determining the main types of bioactive constituents, eighteen in all from the formula, involving flavonoids, saponins, organic acid and some volatile compounds, was developed. This method was based on HPLC coupled to a diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) on a common reverse-phase C(18) column. Liquid chromatography coupled with on-line electrospray ionization mass spectrometry (LC-ESI-MS) was also used to further validate and analyze the constituents. It was found that 0.3% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution. This method, which showed good precision and accuracy, was successfully used to quantify the bioactive constituents in six products. As a result, the validated HPLC method, together with the LC-ESI-MS analysis, provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components"
Keywords:		"Calibration Chromatography, High Pressure Liquid/*methods Drugs, Chinese Herbal Light Plant Extracts/*chemistry Quality Control Reference Standards Reproducibility of Results Scattering, Radiation Spectrometry, Mass, Electrospray Ionization/*methods;"
Notes:		"MedlineYi, Ling Qi, Lian-Wen Li, Ping Ma, Yi-Han Luo, Yong-Jing Li, Hai-Yun eng Research Support, Non-U.S. Gov't Validation Study Germany 2007/07/27 Anal Bioanal Chem. 2007 Sep; 389(2):571-80. doi: 10.1007/s00216-007-1431-8. Epub 2007 Jul 25"