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Mol Microbiol

Title:		An RGS protein regulates the pheromone response in the fission yeast Schizosaccharomyces pombe
Author(s):		Watson P; Davis K; Didmon M; Broad P; Davey J;
Address:		"Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK"
Journal Title:		Mol Microbiol
Year:		1999
Volume:		33
Issue:		3
Page Number:		623 - 634
DOI:		10.1046/j.1365-2958.1999.01510.x
ISSN/ISBN:		0950-382X (Print) 0950-382X (Linking)
Abstract:		"The rate and extent of a cell's response to an extracellular stimulus is influenced by regulators that act on the intracellular signalling machinery. Although not directly involved in propagating the intracellular signal, regulators control the activity of the proteins that transmit the signals. To understand this aspect of cell signalling, we have studied the pheromone response pathway in the fission yeast Schizosaccharomyces pombe, a relatively simple signalling system in a genetically tractable organism. We demonstrate this approach by investigating the role of Rgs1, a member of the Regulator of G protein Signalling (RGS) family of proteins. The rgs1 gene was identified through the Sz. pombe genome sequencing project (accession number Q09777) and recognized as having similarity to RGS proteins [Tesmer et al. (1997) Cell 89: 251-261], but this is the first report concerning the activity of the protein. Strains lacking rgs1 (Deltargs1) are hypersensitive to pheromone stimulation and unable to conjugate with a mating partner. Inhibition of mating occurs at a relative late stage in the process as Deltargs1 strains exhibit pheromone-dependent transcription and form shmoos. Expression of SST2 (an RGS protein that regulates pheromone signalling in the budding yeast Saccharomyces cerevisiae) overcomes the hypersensitivity of the Deltargs1 strains but fails to rescue their mating defect"
Keywords:		"Amino Acid Sequence Fungal Proteins *GTPase-Activating Proteins Gene Expression Regulation, Fungal Genes, Fungal Genes, Reporter Molecular Sequence Data Pheromones/*pharmacology Proteins/*genetics/metabolism *RGS Proteins Saccharomyces cerevisiae/genetics;"
Notes:		"MedlineWatson, P Davis, K Didmon, M Broad, P Davey, J eng Research Support, Non-U.S. Gov't England 1999/07/27 Mol Microbiol. 1999 Aug; 33(3):623-34. doi: 10.1046/j.1365-2958.1999.01510.x"