« Previous AbstractRemoval of volatile organic compounds (VOCs) from waste air stream using ozone assisted zinc oxide (ZnO) nanoparticles coated on zeolite    Next AbstractUse of CLSA and SPME-headspace techniques followed by GC-MS analysis to extract and identify the floral odorants »

Plasmid

Title:		Structural analysis of the Anti-Q-Qs interaction: RNA-mediated regulation of E. faecalis plasmid pCF10 conjugation
Author(s):		Shokeen S; Johnson CM; Greenfield TJ; Manias DA; Dunny GM; Weaver KE;
Address:		"Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, 414 E Clark St. Vermillion, SD 57069, USA"
Journal Title:		Plasmid
Year:		2010
Volume:		20100321
Issue:		1
Page Number:		26 - 35
DOI:		10.1016/j.plasmid.2010.03.002
ISSN/ISBN:		1095-9890 (Electronic) 0147-619X (Print) 0147-619X (Linking)
Abstract:		"Conjugation of the E. faecalis plasmid pCF10 is triggered in response to peptide sex pheromone cCF10 produced by potential recipients. Regulation of this response is complex and multi-layered and includes a small regulatory RNA, Anti-Q that participates in a termination/antitermination decision controlling transcription of the conjugation structural genes. In this study, the secondary structure of the Anti-Q transcript and its sites of interaction with its target, Qs, were determined. The primary site of interaction occurred at a centrally-located loop whose sequence showed high variability in analogous molecules on other pheromone-responsive plasmids. This loop, designated the specificity loop, was demonstrated to be important but not sufficient for distinguishing between Qs molecules from pCF10 and another pheromone-responsive plasmid pAD1. A loop 5' from the specificity loop which carries a U-turn motif played no demonstrable role in Anti-Q-Qs interaction or regulation of the termination/antitermination decision. These results provide direct evidence for a critical role of Anti-Q-Qs interactions in posttranscriptional regulation of pCF10 transfer functions"
Keywords:		"Base Sequence *Conjugation, Genetic Enterococcus faecalis/*genetics Molecular Sequence Data Mutation/genetics Nucleic Acid Conformation Oligopeptides/*genetics Pheromones/*genetics Plasmids/*genetics RNA, Bacterial/*chemistry/genetics Regulatory Sequences;"
Notes:		"MedlineShokeen, Sonia Johnson, Christopher M Greenfield, Tony J Manias, Dawn A Dunny, Gary M Weaver, Keith E eng GM55544/GM/NIGMS NIH HHS/ GM49530/GM/NIGMS NIH HHS/ R01 GM049530/GM/NIGMS NIH HHS/ R01 GM055544/GM/NIGMS NIH HHS/ R01 GM055544-07/GM/NIGMS NIH HHS/ R01 GM049530-25/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2010/03/25 Plasmid. 2010 Jul; 64(1):26-35. doi: 10.1016/j.plasmid.2010.03.002. Epub 2010 Mar 21"