| Title: | The ubiquitin ligase SCFGrr1 is necessary for pheromone sensitivity in Saccharomyces cerevisiae |
| Author(s): | Schweitzer K; Cocklin R; Garrett L; Desai F; Goebl M; |
| Address: | "Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA" |
| ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "The presence of the appropriate pheromone induces alpha and a cells of the yeast Saccharomyces cerevisiae to activate both changes in transcriptional expression and cell polarity that eventually lead to the mating of alpha and a cells to form a/alpha diploid cells. A third response after exposure to mating pheromone is a transient cell cycle arrest, allowing synchronization of the two cell types in G1 prior to cell fusion. At least in part, this cell cycle arrest requires the inactivation of Cln-kinase activity through transcriptional inactivation of the CLN1 and CLN2 genes, degradation of the Cln proteins and direct inhibition of Cln-kinase complexes. Here we report that GRR1, which encodes a substrate recognition subunit of SCF complexes, is critical for pheromone sensitivity and likely for this arrest. Loss of SCF(Grr1) function by deletion of the GRR1 gene causes pheromone resistance. However, deletion of CLN1 and CLN2 restores pheromone sensitivity to grr1Delta cells. Thus, rapid loss of Cln-kinase activity during mating may require coordinated inactivation of the Cln-kinase complexes, inactivation of CLN transcription and SCF(Grr1)-dependent Cln degradation" |
| Keywords: | "Aminopeptidases Cell Cycle/drug effects Cyclins/genetics/metabolism Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Endopeptidases F-Box Proteins *Gene Expression Regulation, Fungal Mutation Peptide Hydrolases/genetics/metabolism Pheromones/*pharmacology;" |
| Notes: | "MedlineSchweitzer, Kelly Cocklin, Ross Garrett, Lisa Desai, Falguni Goebl, Mark eng Research Support, U.S. Gov't, Non-P.H.S. England 2005/06/09 Yeast. 2005 May; 22(7):553-64. doi: 10.1002/yea.1234" |
