| Title: | Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis |
| Author(s): | Savelev SU; Perry JD; Bourke SJ; Jary H; Taylor R; Fisher AJ; Corris PA; Petrie M; De Soyza A; |
| Address: | "Institute of Aging and Health, University of Newcastle, Newcastle upon Tyne, UK" |
| DOI: | 10.1111/j.1472-765X.2011.03049.x |
| ISSN/ISBN: | 1472-765X (Electronic) 0266-8254 (Linking) |
| Abstract: | "AIMS: The purpose of this study was to determine whether volatile organic compounds specific to Pseudomonas aeruginosa could be detected in clinical sputum specimens. METHODS AND RESULTS: Patients were recruited from specialist bronchiectasis and cystic fibrosis clinics. The gold standard for diagnosing Ps. aeruginosa infection was a positive sputum culture. About 72 sputum headspace samples taken from patients at risk of or known to have prior Ps. aeruginosa infection were analysed by solid phase micro-extraction mass spectrometry. 2-nonanone was a marker in Ps. aeruginosa in sputum headspace gas with sensitivity of 72% and specificity of 88%. A combination of volatile compounds, a sputum library of 17 compounds with 2-nonanone, increased sensitivity in the detection of Ps. aeruginosa to 91% with specificity of 88%. CONCLUSIONS: In contrast to the 48-hour turnaround for classical microbiological culture, these results were available within 1-2 h. These data demonstrate the potential for rapid and accurate diagnosis of Ps. aeruginosa infection from sputum samples. SIGNIFICANCE AND IMPACT OF THE STUDY: 2-Nonanone is a compound requiring further study in the exhaled breath as it may improve diagnostic of Ps. aeruginosa infection when combined with other reported volatile markers" |
| Keywords: | "Adult Biomarkers/analysis Bronchiectasis/*microbiology Chromatography, Gas/methods Cystic Fibrosis/*microbiology *Diagnostic Techniques and Procedures Humans Ketones/analysis Pseudomonas Infections/*diagnosis Pseudomonas aeruginosa/*isolation & purificati;" |
| Notes: | "MedlineSavelev, S U Perry, J D Bourke, S J Jary, H Taylor, R Fisher, A J Corris, P A Petrie, M De Soyza, A eng Evaluation Study Research Support, Non-U.S. Gov't England 2011/03/29 Lett Appl Microbiol. 2011 Jun; 52(6):610-3. doi: 10.1111/j.1472-765X.2011.03049.x. Epub 2011 Apr 14" |
