| Title: | Using mass spectrometry to detect buffalo salivary odorant-binding protein and its post-translational modifications |
| Author(s): | Rajkumar R; Karthikeyan K; Archunan G; Huang PH; Chen YW; Ng WV; Liao CC; |
| Address: | "Center for Pheromone Technology, Department of Animal Science, Bharathidasan University, Trichirappalli 620 024, Tamilnadu, India" |
| Journal Title: | Rapid Commun Mass Spectrom |
| ISSN/ISBN: | 1097-0231 (Electronic) 0951-4198 (Linking) |
| Abstract: | "A large number of mammalian odorant-binding proteins, which are lipocalins, have been studied. These proteins participate in peri-receptor events by selecting and carrying odorant molecules. The present study aimed at identifying the buffalo salivary odorant-binding protein (sOBP), and to determine its post-translational modification using mass spectrometry. The buffalo salivary 21 kDa protein was initially separated adopting sodium dodecyl sulfate-polyacrylamide gel electrophoresis and it was identified as sOBP with high statistical reliability using liquid chromatography/tandem mass spectrometry (LC/MS/MS) and SEQUEST, for the first time. Further, the post-translationally modified peptides were screened adopting MS/MS. A total of four post-translational modifications, namely glycation at lysine-(59), hydroxylation at lysine-(134), ubiquitination at lysine-(121), and dihydroxylation in lysine-(108), were recorded. Moreover, these modifications have not been identified in buffalo salivary odorant-binding protein" |
| Keywords: | "Amino Acid Sequence Animals *Buffaloes Chromatography, Liquid Electrophoresis, Polyacrylamide Gel Female Glycoproteins/chemistry/metabolism Glycosylation Hydroxylation Lysine/chemistry/metabolism Models, Molecular Molecular Sequence Data Periodic Acid-Sch;" |
| Notes: | "MedlineRajkumar, R Karthikeyan, K Archunan, G Huang, P H Chen, Y W Ng, W V Liao, C C eng Research Support, Non-U.S. Gov't England 2010/10/26 Rapid Commun Mass Spectrom. 2010 Nov 30; 24(22):3248-54. doi: 10.1002/rcm.4766" |
