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Chem Senses

Title:		Unisex pheromone detectors and pheromone-binding proteins in scarab beetles
Author(s):		Nikonov AA; Peng G; Tsurupa G; Leal WS;
Address:		"Laboratory of Chemical Prospecting, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba 305-8634, Japan"
Journal Title:		Chem Senses
Year:		2002
Volume:		27
Issue:		6
Page Number:		495 - 504
DOI:		10.1093/chemse/27.6.495
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"Olfaction was studied in two species of scarab beetle, Anomala octiescostata and Anomala cuprea (Coleoptera: Scarabaeidae: Rutelinae), which are temporarily isolated and use the same sex pheromone compounds, (R)-buibuilactone and (R)-japonilure. Single sensillum recordings in A. octiescostata revealed highly sensitive olfactory receptor neurons (ORNs) (threshold <1 pg) that were tuned to the detection of the green leaf volatile compound (Z)-3-hexenyl acetate. As opposed to similar ORNs in another scarab species, Phyllopertha diversa, in A. octiescostata a diazo analogue elicited much lower neuronal responses than the natural ligand. Detectors for other floral and leaf compounds were also characterized. Extremely stereoselective ORNs tuned to sex pheromone were identified in male and female antennae. Biochemical investigations showed that, in A. octiescostata and A. cuprea, the pheromone-binding proteins (PBPs) isolated from male antennae were identical to PBPs obtained from female antennae. AoctPBP and AcupPBP had seven different amino acid residues. Binding of AoctPBP to (R)-japonilure is shown. PdivOBP1, which is also known to bind to (R)-japonilure, differed from AcupPBP in only two amino acid residues, one at the N-terminus and the other near the C-terminus. The structural features of the Bombyx mori PBP are compared with the sequences of eight known scarab odorant-binding proteins"
Keywords:		"Acetates/pharmacology Amino Acid Sequence Animals Bombyx/chemistry Carrier Proteins/chemistry/*metabolism Coleoptera/*physiology Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel/methods Female *Insect Proteins Male Mass Spectrometry Mo;"
Notes:		"MedlineNikonov, Alexander Alexeevich Peng, Guihong Tsurupa, Galina Leal, Walter Soares eng Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2002/07/27 Chem Senses. 2002 Jul; 27(6):495-504. doi: 10.1093/chemse/27.6.495"