| Title: | "Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding" |
| Author(s): | Medeiros AH; Mingossi FB; Dias RO; Franco FP; Vicentini R; Mello MO; Moura DS; Silva-Filho MC; |
| Address: | "Departamento de Ciencias da Natureza, Matematica e Educacao, Universidade Federal de Sao Carlos, Araras, 13600-970 Sao Paulo, Brazil. anehm@cca.ufscar.br. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. anehm@cca.ufscar.br. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. fabiana.mingossi@gmail.com. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. diasrdo@gmail.com. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. flavia.esalq@gmail.com. Systems Biology Laboratory, Center for Molecular Biology and Genetic Engineering, State University of Campinas, Campinas, 13083-970 Sao Paulo, Brazil. shinapes@unicamp.br. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. marcia.o.jose@gmail.com. Monsanto do Brasil, Campinas, 13069-380 Sao Paulo, Brazil. marcia.o.jose@gmail.com. Departamento de Ciencias Biologicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13400-918 Sao Paulo, Brazil. danielmoura@usp.br. Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, 13418-260 Sao Paulo, Brazil. mdcsilva@usp.br" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Sugarcane's (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory" |
| Keywords: | Animals Down-Regulation Endopeptidase Clp/genetics/*metabolism Host-Parasite Interactions/*genetics Lepidoptera/*pathogenicity Phylogeny Plant Proteins/genetics/*metabolism Protein Subunits/genetics/metabolism Saccharum/*enzymology/genetics/parasitology S; |
| Notes: | "MedlineMedeiros, Ane H Mingossi, Fabiana B Dias, Renata O Franco, Flavia P Vicentini, Renato Mello, Marcia O Moura, Daniel S Silva-Filho, Marcio C eng Switzerland 2016/09/07 Int J Mol Sci. 2016 Sep 1; 17(9):1444. doi: 10.3390/ijms17091444" |
