| Title: | "Voxel-based analysis of the immediate early gene, c-jun, in the honey bee brain after a sucrose stimulus" |
| Address: | "Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA; Neuroscience Program, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA" |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "Immediate early genes (IEGs) have served as useful markers of brain neuronal activity in mammals, and more recently in insects. The mammalian canonical IEG, c-jun, is part of regulatory pathways conserved in insects and has been shown to be responsive to alarm pheromone in honey bees. We tested whether c-jun was responsive in honey bees to another behaviourally relevant stimulus, sucrose, in order to further identify the brain regions involved in sucrose processing. To identify responsive regions, we developed a new method of voxel-based analysis of c-jun mRNA expression. We found that c-jun is expressed in somata throughout the brain. It was rapidly induced in response to sucrose stimuli, and it responded in somata near the antennal and mechanosensory motor centre, mushroom body calices and lateral protocerebrum, which are known to be involved in sucrose processing. c-jun also responded to sucrose in somata near the lateral suboesophageal ganglion, dorsal optic lobe, ventral optic lobe and dorsal posterior protocerebrum, which had not been previously identified by other methods. These results demonstrate the utility of voxel-based analysis of mRNA expression in the insect brain" |
| Keywords: | "Animals Bees/genetics/*metabolism Brain/metabolism *Genes, Immediate-Early *Genes, jun Mushroom Bodies/metabolism Organ Specificity Sucrose/*pharmacology brain c-jun confocal microscope honey bee immediate early gene mRNA in situ hybridization proboscis e;Neuroscience;" |
| Notes: | "MedlineMcNeill, M S Robinson, G E eng England 2015/03/17 Insect Mol Biol. 2015 Jun; 24(3):377-90. doi: 10.1111/imb.12165. Epub 2015 Mar 14" |
