| Title: | Emerging Molecular Tools for Engineering Phytomicrobiome |
| Author(s): | Chaudhary T; Gera R; Shukla P; |
| Address: | "Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001 India. GRID: grid.411524.7. ISNI: 0000 0004 1790 2262 Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004 India. GRID: grid.7151.2. ISNI: 0000 0001 0170 2635 Present Address: School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005 India. GRID: grid.411507.6. ISNI: 0000 0001 2287 8816" |
| DOI: | 10.1007/s12088-020-00915-1 |
| ISSN/ISBN: | 0046-8991 (Print) 0973-7715 (Electronic) 0046-8991 (Linking) |
| Abstract: | "Microbial plant interaction plays a major role in the sustainability of plants. The understanding of phytomicrobiome interactions enables the gene-editing tools for the construction of the microbial consortia. In this interaction, microbes share several common secondary metabolites and terpenoid metabolic pathways with their host plants that ensure a direct connection between the microbiome and associated plant metabolome. In this way, the CRISPR-mediated gene-editing tool provides an attractive approach to accomplish the creation of microbial consortia. On the other hand, the genetic manipulation of the host plant with the help of CRISPR-Cas9 can facilitate the characterization and identification of the genetic determinants. It leads to the enhancement of microbial capacity for more trait improvement. Many plant characteristics like phytovolatilization, phytoextraction, phytodesalination and phytodegradation are targeted by these approaches. Alternatively, chemical communications by PGPB are accomplished by the exchange of different signal molecules. For example, quorum-sensing is the way of the cell to cell communication in bacteria that lead to the detection of metabolites produced by pathogens during adverse conditions and also helpful in devising some tactics towards understanding plant immunity. Along with quorum-sensing, different volatile organic compounds and N-acyl homoserine lactones play a significant role in cell to cell communication by microbe to plant and among the plants respectively. Therefore, it is necessary to get details of all the significant approaches that are useful in exploring cell to cell communications. In this review, we have described gene-editing tools and the cell to cell communication process by quorum-sensing based signaling. These signaling processes via CRISPR- Cas9 mediated gene editing can improve the microbe-plant community in adverse climatic conditions" |
| Keywords: | Cell communication Microbial community Phytomicrobiome Plant-microbe interactions Quorum-sensing; |
| Notes: | "PubMed-not-MEDLINEChaudhary, Twinkle Gera, Rajesh Shukla, Pratyoosh eng Review India 2021/05/01 Indian J Microbiol. 2021 Jun; 61(2):116-124. doi: 10.1007/s12088-020-00915-1. Epub 2021 Jan 18" |
