| Title: | "PhERF6, interacting with EOBI, negatively regulates fragrance biosynthesis in petunia flowers" |
| Author(s): | Liu F; Xiao Z; Yang L; Chen Q; Shao L; Liu J; Yu Y; |
| Address: | "Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. College of Horticulture, South China Agricultural University, Guangzhou, 510642, China" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "In petunia, the production of volatile benzenoids/phenylpropanoids determines floral aroma, highly regulated by development, rhythm and ethylene. Previous studies identified several R2R3-type MYB trans-factors as positive regulators of scent biosynthesis in petunia flowers. Ethylene response factors (ERFs) have been shown to take part in the signal transduction of hormones, and regulation of metabolism and development processes in various plant species. Using virus-induced gene silencing technology, a negative regulator of volatile benzenoid biosynthesis, PhERF6, was identified by a screen for regulators of the expression of genes related to scent production. PhERF6 expression was temporally and spatially connected with scent production and was upregulated by exogenous ethylene. Up-/downregulation of the mRNA level of PhERF6 affected the expression of ODO1 and several floral scent-related genes. PhERF6 silencing led to a significant increase in the concentrations of volatiles emitted by flowers. Yeast two-hybrid, bimolecular fluorescence complementation and co-immunoprecipitation assays indicated that PhERF6 interacted with the N-terminus of EOBI, which includes two DNA binding domains. Our results show that PhERF6 negatively regulates volatile production in petunia flowers by competing for the binding of the c-myb domains of the EOBI protein with the promoters of genes related to floral scent" |
| Keywords: | "Cell Nucleus/metabolism Down-Regulation/genetics Electrophoretic Mobility Shift Assay Ethylenes/metabolism Flowers/genetics/*metabolism Fluorescence Gene Expression Regulation, Plant Gene Silencing Genes, Plant Odorants Petunia/genetics/*metabolism Plant;" |
| Notes: | "MedlineLiu, Fei Xiao, Zhina Yang, Li Chen, Qian Shao, Lu Liu, Juanxu Yu, Yixun eng England 2017/07/05 New Phytol. 2017 Sep; 215(4):1490-1502. doi: 10.1111/nph.14675. Epub 2017 Jul 4" |
