« Previous AbstractPhytohormone ecology : Herbivory byThrips tabaci induces greater ethylene production in intact onions than mechanical damage alone    Next AbstractResponse of Anastrepha suspensa (Diptera: Tephritidae) to terminal diamines in a food-based synthetic attractant »

J Chromatogr A

Title:		Target list building for volatile metabolite profiling of fruit
Author(s):		Kende A; Portwood D; Senior A; Earll M; Bolygo E; Seymour M;
Address:		"Analytical Sciences, Syngenta Jealott's Hill International Research Centre, Jealott's Hill, Bracknell, Berkshire, UK. aniko.kende@syngenta.com"
Journal Title:		J Chromatogr A
Year:		2010
Volume:		20100608
Issue:		43
Page Number:		6718 - 6723
DOI:		10.1016/j.chroma.2010.05.030
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"Fruit flavour is the combination of numerous biochemicals: sugars for sweetness, acids for sourness and volatile metabolites for aroma. The objective of this study was to establish a method to develop a target list of statistically relevant compounds for the characterization of melon from non-targeted data, while preserving the profile information. Five different varieties were sampled (sampling 12 biological replicates from 12 plants) using dynamic headspace extraction, then analysed by gas chromatography-mass spectrometry in full scan mode. Using Metalign and SIMCA-P software the raw data was spectrally aligned and then subjected to principal component analysis (PCA). The principal component analysis plot showed good separation of the five varieties based on their full scan GC-MS profile. Mass spectral data points responsible for the differences between varieties were highlighted by further statistical analysis. The mass spectra were then reconstructed and the corresponding chemicals identified using library search or reference standards were available to create a new target component list. To validate the new target list, the initial data set was re-processed using the targeted approach and the results subjected again to principal component analysis. The two representations showed excellent agreement on the separation of the five varieties. The new target list obtained from this study can be applied to differentiate and characterize the volatile profile of melon varieties using a list of statistically significant compounds"
Keywords:		Cucurbitaceae/*chemistry Fruit/*chemistry Gas Chromatography-Mass Spectrometry Metabolome Metabolomics/methods Multivariate Analysis Odorants/*analysis Principal Component Analysis Reproducibility of Results Species Specificity Volatile Organic Compounds/;
Notes:		"MedlineKende, Aniko Portwood, David Senior, Adam Earll, Mark Bolygo, Elek Seymour, Mark eng Netherlands 2010/07/06 J Chromatogr A. 2010 Oct 22; 1217(43):6718-23. doi: 10.1016/j.chroma.2010.05.030. Epub 2010 Jun 8"