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Bio Protoc

Title:		An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
Author(s):		Cecchini NM; Song Y; Roychoudhry S; Greenberg JT; Haney CH;
Address:		"Centro de Investigaciones en Quimica Biologica de Cordoba (CIQUIBIC), CONICET, Departamento de Quimica Biologica-Ranwel Caputto, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Haya de la Torre y Medina Allende, Cordoba X5000HUA, Argentina. Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK. Department of Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS 524W, Chicago, IL 60637, USA"
Journal Title:		Bio Protoc
Year:		2019
Volume:		20190520
Issue:		10
Page Number:		e3236 -
DOI:		10.21769/BioProtoc.3236
ISSN/ISBN:		2331-8325 (Electronic) 2331-8325 (Linking)
Abstract:		"The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas spp. with the reference plant Arabidopsis thaliana. Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen Pseudomonas cannabina pv. alisalensis (formerly called Pseudomonas syringae pv. maculicola) strain ES4326 and the herbivore Trichoplusia ni by inoculating Pseudomonas simiae strain WCS417 (formerly called Pseudomonas fluorescens WCS417) on Arabidopsis plants grown in Jiffy-7((R)) peat pellets. While most commonly used for Pseudomonas-triggered ISR on Arabidopsis, this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores"
Keywords:		Arabidopsis thaliana Induced Systemic Resistance (ISR) Pseudomonas cannabina Pseudomonas simiae Pseudomonas syringae Trichoplusia ni;
Notes:		"PubMed-not-MEDLINECecchini, Nicolas M Song, Yi Roychoudhry, Suruchi Greenberg, Jean T Haney, Cara H eng 2019/05/20 Bio Protoc. 2019 May 20; 9(10):e3236. doi: 10.21769/BioProtoc.3236. eCollection 2019 May 20"