« Previous AbstractReview: Allelochemicals as multi-kingdom plant defence compounds: towards an integrated approach    Next AbstractScience and Technology Solutions for Scalable SARS-CoV-2 Testing to Inform Return to Full Capacity Strategy in United States Air Force Workforce Personnel »

Sci Rep

Title:		Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors
Author(s):		Hickner PV; Mittapalli O; Subramoniam A; Sagel A; Watson W; Scott MJ; Arp AP; de Leon AAP; Syed Z;
Address:		"Department of Entomology, University of Kentucky, Lexington, KY, 40546, USA. USDA, Agricultural Research Service, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama. Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, 27695-7613, USA. USDA, Agricultural Research Service, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA. Department of Entomology, University of Kentucky, Lexington, KY, 40546, USA. zsy224@uky.edu"
Journal Title:		Sci Rep
Year:		2020
Volume:		20201127
Issue:		1
Page Number:		20771 -
DOI:		10.1038/s41598-020-77541-w
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"The screwworm fly, Cochliomyia hominivorax (Coquerel), was successfully eradicated from the United States by the sterile insect technique (SIT). However, recent detection of these flies in the Florida Keys, and increased risk of introductions to the other areas warrant novel tools for management of the flies. Surveillance, a key component of screwworm control programs, utilizes traps baited with rotting liver or a blend of synthetic chemicals such as swormlure-4. In this work, we evaluated the olfactory physiology of the screwworm fly and compared it with the non-obligate ectoparasitic secondary screwworm flies, C. macellaria, that invade necrotic wound and feed on dead tissue. These two species occur in geographically overlapping regions. C. macellaria, along with other blowflies such as the exotic C. megacephala, greatly outnumber C. hominivorax in the existing monitoring traps. Olfactory responses to swormlure-4 constituents between sex and mating status (mated vs unmated) in both species were recorded and compared. Overall, responses measured by the antennograms offered insights into the comparative olfactory physiology of the two fly species. We also present detailed analyses of the antennal transcriptome by RNA-Sequencing that reveal significant differences between male and female screwworm flies. The differential expression patterns were confirmed by quantitative PCR. Taken together, this integrated study provides insights into the physiological and molecular correlates of the screwworm's attraction to wounds, and identifies molecular targets that will aid in the development of odorant-based fly management strategies"
Keywords:		"Animals Behavior, Animal Diptera/classification/drug effects/*physiology Female Gene Expression Regulation Male Odorants/*analysis Pheromones/*metabolism/pharmacology RNA-Seq Wounds and Injuries/*metabolism;"
Notes:		"MedlineHickner, Paul V Mittapalli, Omprakash Subramoniam, Anjana Sagel, Agustin Watson, Wes Scott, Maxwell J Arp, Alex P de Leon, Adalberto A Perez Syed, Zainulabeuddin eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2020/11/29 Sci Rep. 2020 Nov 27; 10(1):20771. doi: 10.1038/s41598-020-77541-w"