| Title: | "Purification and partial amino acid sequence analysis of the larval settlement-inducing pheromone from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite)" |
| Author(s): | Endo N; Nogata Y; Yoshimura E; Matsumura K; |
| Address: | "Central Research Institute of Electric Power Industry, Abiko-shi, Chiba-ken, Japan. n-endo@criepi.denken.or.jp" |
| DOI: | 10.1080/08927010902875113 |
| ISSN/ISBN: | 1029-2454 (Electronic) 0892-7014 (Linking) |
| Abstract: | "A previously undescribed larval settlement-inducing protein was purified from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite). Results of SDS-PAGE indicated that the relative molecular mass of the protein in reduced and denatured form is 31,600 +/- 500 kDa, and that it is distinct from the Settlement Inducing Protein Complex (SIPC) which has previously been determined as a larval settlement-inducing pheromone. The N-terminal 33-residue sequence of the intact protein showed no similarity with previously reported proteins in the EMBL/Genbank/DDBJ databases. The purified protein at a concentration of 10 microg ml(-1) induced approximately four times more larval settlement than the control (filtered natural seawater). In addition, results of the assay using both 24-well polystyrene plates and agarose gels indicated that this protein is probably released into seawater and attracts cypris larvae. These results suggest that the purified protein is a waterborne type pheromone which induces settlement of larvae of B. amphitrite" |
| Keywords: | "Amino Acid Sequence Animals *Behavior, Animal Electrophoresis, Polyacrylamide Gel Larva/growth & development Molecular Sequence Data Molecular Weight Pheromones/chemistry/*isolation & purification Thoracica/*growth & development/metabolism;" |
| Notes: | "MedlineEndo, Noriyuki Nogata, Yasuyuki Yoshimura, Erina Matsumura, Kiyotaka eng England 2009/03/27 Biofouling. 2009; 25(5):429-34. doi: 10.1080/08927010902875113" |
