| Title: | Protein-peptide nutritional material prepared from surimi wash-water using immobilized chymotrypsin-trypsin |
| Author(s): | Ding HC; Li DF; Wei XY; Huang YW; Cui S; Xie HJ; Zhou T; |
| Address: | "School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China. Food and Drug Testing Institute of Zhoushan, Zhejiang, 316000, China. Department of Food, Faculty of Hotel Management, Shanghai Business School, Shanghai, 200235, China. Department of Food Science and Technology, University of Georgia, Athens, GA, 30602, USA" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: In the production process of surimi, large quantities of wastewater are produced. Thus it would be interesting to develop an efficient protocol for the recovery of protein from hairtail surimi wash-water. RESULTS: A technique involving the use of immobilized chymotrypsin-trypsin (I-CT) was developed, providing a practical method for the preparation of protein-peptide nutritional material (PPNM). Under optimized reaction conditions, the recovery rate of nitrogen of surimi wash-water was measured as 98.3 +/- 2.9%. Nutritional evaluation of the protein-peptide fraction demonstrated that it contained all essential amino acids (EAA) for humans, accounting for 44.1% of the total amino acid (TAA) content, which was determined to be 78.2 g per 100 g dry matter. The essential amino acid index (EAAI) and biological value (BV) were 101.7 (>95) and 76.7 respectively. A wide range of volatile flavor compounds (>50), including aldehydes, ketones, alcohols, hydrocarbons and heterocyclic compounds, were identified in PPNM by gas chromatography/mass spectrometry (GC/MS) analysis. CONCLUSION: An efficient and practical protocol for the recovery of protein from hairtail surimi wash-water has been developed. The PPNM prepared in this work could be used as a nutraceutical and as an ingredient of functional foods. (c) 2016 Society of Chemical Industry" |
| Keywords: | "Biocatalysis Chymotrypsin/*chemistry Enzymes, Immobilized/chemistry Fish Products/*analysis Fish Proteins/*chemistry Gas Chromatography-Mass Spectrometry Peptides/*chemistry Trypsin/*chemistry Volatile Organic Compounds/chemistry Waste Products/analysis W;" |
| Notes: | "MedlineDing, Hao-Chen Li, Dong-Fang Wei, Xiao-Yi Huang, Yao-Wen Cui, Shuai Xie, Hu-Jun Zhou, Tao eng England 2016/07/29 J Sci Food Agric. 2017 Apr; 97(6):1746-1752. doi: 10.1002/jsfa.7969. Epub 2016 Aug 18" |
