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Ann Agric Environ Med

Title:		Volatile profiles and aflatoxin production by toxigenic and non-toxigenic isolates of Aspergillus flavus grown on sterile and non-sterile cracked corn
Author(s):		De Lucca AJ; Boue SM; Carter-Wientjes C; Bhatnagar D;
Address:		"Southern Regional Research Center, USDA, New Orleans, LA, USA. Anthony.DeLucca@ars.usda.gov"
Journal Title:		Ann Agric Environ Med
Year:		2012
Volume:		19
Issue:		1
Page Number:		91 - 98
DOI:
ISSN/ISBN:		1898-2263 (Electronic) 1232-1966 (Linking)
Abstract:		"Aspergillus flavus is a saprophytic fungus which can grow on corn and produce aflatoxins which render it unsafe for consumption as food and feed. In this study, aflatoxin and non-aflatoxin producing isolates of A. flavus were grown separately on wet (20% water added), sterile or non-sterile cracked corn. Wet and dry cracked corn controls were included as needed. Secondary metabolic volatiles were identified and aflatoxin concentrations determined over a 12-day period. Volatiles unique to the toxigenic A. flavus isolates were determined by comparison with volatiles produced by the respective corn controls and the non-toxigenic A. flavus isolate. The number and identity of the volatiles produced by these A. flavus isolates varied by isolate, whether sterile or non-sterile corn was the substrate, and the sampling day. Overall, most of the volatiles were produced before day 8 after inoculation. Aflatoxin production was 10-fold lower on the sterile corn, compared to the non-sterile corn. Volatiles unique to the aflatoxin producing isolates were identified on both substrates after comparison with those produced by the non-aflatoxin producing isolate, as well as the corn control samples. Results indicate that several factors (substrate, fungal isolate, culture age) affect volatile and aflatoxin production by A. flavus"
Keywords:		"Aflatoxin B1/*biosynthesis Aspergillus flavus/*chemistry/classification/isolation & purification Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Polymers/chemistry Species Specificity Time Factors Volatile Organic Compounds/*anal;"
Notes:		"MedlineDe Lucca, Anthony J Boue, Stephen M Carter-Wientjes, Carol Bhatnagar, Deepak eng Poland 2012/04/03 Ann Agric Environ Med. 2012; 19(1):91-8"