| Title: | Yeast alpha-mating factor receptor-linked G-protein signal transduction suppresses Ras-dependent activity |
| Author(s): | Arkinstall SJ; Papasavvas SG; Payton MA; |
| Address: | "Department of Biological Chemistry, Glaxo Institute for Molecular Biology, Geneva, Switzerland" |
| DOI: | 10.1016/0014-5793(91)80777-z |
| ISSN/ISBN: | 0014-5793 (Print) 0014-5793 (Linking) |
| Abstract: | "Homologues of mammalian Ras conserved in Saccharomyces cerevisiae mediate glucose-stimulated cyclic AMP formation and we used this response to test for regulation of yeast Ras activity by the alpha-mating factor signal transduction pathway. alpha-Mating factor suppresses glucose-stimulated cyclic AMP formation by up to 57 +/- 12.6% (n = 5) and similar inhibition was observed in four different yeast strains (MATa cells). Moreover, this response is potent (IC50 = 0.14 +/- 0.19 microM (n = 4)), rapid (maximal within 1-2 min), and displays an absolute requirement for both the alpha-mating factor receptor (STE2) and associated G-protein beta-subunit (STE4). Inhibition appears independent of both phosphodiesterase activation and alpha-mating factor-stimulated cytoplasmic alkalinization. Also, basal cyclic AMP levels are unaffected by pheromone. This is the first demonstration that a cell-surface receptor linked to a heterotrimeric G-protein can suppress Ras-dependent activity and could provide important insight into mechanisms controlling p21ras in man. Inhibition of Ras-dependent cyclic AMP formation could also be a key event facilitating responses characteristic of yeast mating" |
| Keywords: | "Cyclic AMP/metabolism GTP-Binding Proteins/*metabolism Gene Expression Regulation, Fungal Genes, Fungal *Genes, ras Kinetics Mating Factor Peptides/metabolism Pheromones/metabolism Receptors, Cell Surface/*metabolism Receptors, Mating Factor *Receptors, P;" |
| Notes: | "MedlineArkinstall, S J Papasavvas, S G Payton, M A eng England 1991/06/17 FEBS Lett. 1991 Jun 17; 284(1):123-8. doi: 10.1016/0014-5793(91)80777-z" |
