| Title: | Real-time monitoring of skin ethanol gas by a high-sensitivity gas phase biosensor (bio-sniffer) for the non-invasive evaluation of volatile blood compounds |
| Author(s): | Arakawa T; Suzuki T; Tsujii M; Iitani K; Chien PJ; Ye M; Toma K; Iwasaki Y; Mitsubayashi K; |
| Address: | "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka, Japan. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: m.bdi@tmd.ac.jp" |
| DOI: | 10.1016/j.bios.2018.09.070 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Linking) |
| Abstract: | "In this study, a highly sensitive and selective biochemical gas sensor (bio-sniffer) and real-time monitoring system with skin gas cell was constructed for the determination of ethanol gas concentration on human skin. This bio-sniffer measured the concentration of ethanol according to the change in fluorescence intensity of nicotinamide adenine dinucleotide (NADH), which is produced in an enzymatic reaction by alcohol dehydrogenase (ADH). The NADH detection system used an ultraviolet light emitting diode (UV-LED) as the excitation light, and a highly sensitive photomultiplier tube as a fluorescence intensity detector. The calibration range of the ethanol bio-sniffer was validated from 25 ppb??? to 128 ppm???. To measure the concentration of ethanol within skin gas, subjects ingested an alcohol beverage, and the sensor output was monitored. We chose the central part of the palm, a back of the hand, and a wrist as targets. The real-time concentration of skin ethanol gas at each target was measured after drinking. The maximum output values were reached at approximately 70?ª+min after drinking and then gradually decreased. We showed that ethanol release kinetics were different depending on the part of the hand measured with the developed monitoring system. Accordingly, this highly sensitive and selective bio-sniffer with a skin gas cell could be used to measure ethanol on the skin surface and could be applied for breath and skin gas research, as well as investigation of volatile blood compounds used as biomarkers for clinical diagnosis" |
| Keywords: | Acetaldehyde/chemistry Alcohol Dehydrogenase/chemistry Biosensing Techniques/*instrumentation Breath Tests Equipment Design Ethanol/*analysis Fluorescence Humans Kinetics Limit of Detection NAD/analysis Skin/*chemistry Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineArakawa, Takahiro Suzuki, Takuma Tsujii, Masato Iitani, Kenta Chien, Po-Jen Ye, Ming Toma, Koji Iwasaki, Yasuhiko Mitsubayashi, Kohji eng Validation Study England 2018/10/23 Biosens Bioelectron. 2019 Mar 15; 129:245-253. doi: 10.1016/j.bios.2018.09.070. Epub 2018 Sep 20" |
