| Title: | Investigation of lung cancer biomarkers by hyphenated separation techniques and chemometrics |
| Author(s): | Buszewski B; Ulanowska A; Kowalkowski T; Cieslinski K; |
| Address: | "Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. bbusz@chem.umk.pl" |
| ISSN/ISBN: | 1437-4331 (Electronic) 1434-6621 (Linking) |
| Abstract: | "BACKGROUND: Volatile organic compounds (VOCs) present in breath of lung cancer patients and released by lung cancer cells were determined. As a reference sample breath collected from healthy volunteers and lung segments of cells with proper morphology were used. METHODS: Solid phase microextraction and gas chromato-graphy mass spectrometry (SPME-GC/MS) method was applied for VOC analysis. Statistical methods have been developed to find similarities between patterns of investigated compounds within three populations of samples: exhaled air samples from patients with lung cancer, headspace of lung tissues with healthy and cancer cells. RESULTS: Quantitative analysis of VOCs in the headspace of lung tissues revealed that cancer cells released higher concentrations of ethanol, acetone, carbon disulfide, dimethyl sulfide, 1-propanol, 2-propanol, 2-butanone and 2-pentanone than healthy tissues. The increase of concentration of the same compounds was observed in the breath of patients with lung cancer in comparison to breath from healthy non-smoking volunteers. CONCLUSIONS: Detection of lung cancer is possible by volatile biomarkers analysis in breath" |
| Keywords: | "Biomarkers, Tumor/*analysis/isolation & purification Breath Tests Case-Control Studies Discriminant Analysis Exhalation Female Gas Chromatography-Mass Spectrometry/*methods Humans Informatics/*methods Lung Neoplasms/*chemistry/diagnosis/pathology/physiopa;" |
| Notes: | "MedlineBuszewski, Boguslaw Ulanowska, Agnieszka Kowalkowski, Tomasz Cieslinski, Krzysztof eng Research Support, Non-U.S. Gov't Germany 2011/11/01 Clin Chem Lab Med. 2011 Oct 28; 50(3):573-81. doi: 10.1515/CCLM.2011.769" |
