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Pestic Biochem Physiol

Title:		"Different binding properties of two general-odorant binding proteins in Athetis lepigone with sex pheromones, host plant volatiles and insecticides"
Author(s):		Zhang XQ; Yan Q; Li LL; Xu JW; Mang D; Wang XL; Hoh HH; Ye J; Ju Q; Ma Y; Liang M; Zhang YY; Zhu XY; Zhang F; Dong SL; Zhang YN; Zhang LW;
Address:		"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China; Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China. Bioinspired Soft Matter Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan. Dangshan Plant Protection and Plant Inspection Service Center, Dangshan, China. Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China. Shandong Peanut Research Institute, Qingdao, China. Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China. Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei, China. Electronic address: ynzhang_insect@163.com. Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China. Electronic address: zhanglw@ahau.edu.cn"
Journal Title:		Pestic Biochem Physiol
Year:		2020
Volume:		20200123
Issue:
Page Number:		173 - 182
DOI:		10.1016/j.pestbp.2020.01.012
ISSN/ISBN:		1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:		"Athetis lepigone (Alep) is a polyphagous pest native to Europe and Asia that has experienced major outbreaks in the summer maize area of China since 2011 and has shown evidence of resistance to some insecticides. Insect olfaction is crucial for recognition of sex pheromones, host plant volatiles and even insecticides, in which two general-odorant binding proteins (GOBPs) play important roles. To elucidate the functions of GOBPs in A. lepigone, we first expressed the two AlepGOBP proteins in the E. coli expression system. Then, the results of fluorescence competitive binding assays demonstrated that the high binding affinity of AlepGOBP2 with sex pheromones [(Z)-7-dodecenyl acetate (Z7-12:Ac), Ki = 0.65 muM; (Z)-9-tetradecenyl acetate (Z9-14:Ac), Ki = 0.83 muM], two maize plant volatiles [Ocimene, Ki = 9.63 muM; (E)-beta-Farnesene, Ki = 4.76 muM] and two insecticides (Chlorpyrifos Ki =5.61 muM; Phoxim, Ki = 4.38 muM). However, AlepGOBP1 could only bind Ocimene (Ki = 13.0 muM) and two insecticides (Chlorpyrifos Ki =4.46 muM; Phoxim, Ki = 3.27 muM). These results clearly suggest that AlepGOBP1 and AlepGOBP2 differentiate among odorants and other ligands. The molecular docking results further revealed different key residues involved in the ligand binding of AlepGOBPs. In summary, this study provides a foundation for exploring the olfactory mechanism of A. lepigone and identified two potential target genes for the development of highly effective insecticides in the future"
Keywords:		Animals China Escherichia coli Insect Proteins *Insecticides Molecular Docking Simulation *Moths Odorants Pheromones *Sex Attractants Fluorescence binding assay General-odorant binding protein Molecular docking Tobacco cutworm;
Notes:		"MedlineZhang, Xiao-Qing Yan, Qi Li, Lu-Lu Xu, Ji-Wei Mang, Dingze Wang, Xue-Liang Hoh, Hong-Huat Ye, Jia Ju, Qian Ma, Yu Liang, Meng Zhang, Yun-Ying Zhu, Xiu-Yun Zhang, Fan Dong, Shuang-Lin Zhang, Ya-Nan Zhang, Long-Wa eng 2020/04/15 Pestic Biochem Physiol. 2020 Mar; 164:173-182. doi: 10.1016/j.pestbp.2020.01.012. Epub 2020 Jan 23"