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Pestic Biochem Physiol

Title:		Antifungal effect of volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 on oxidative stress and mitochondrial dysfunction of Ceratocystis fimbriata
Author(s):		Zhang Y; Li T; Xu M; Guo J; Zhang C; Feng Z; Peng X; Li Z; Xing K; Qin S;
Address:		"School of Life Science, the Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, PR China. School of Life Science, the Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, PR China. Electronic address: xingke@jsnu.edu.cn"
Journal Title:		Pestic Biochem Physiol
Year:		2021
Volume:		20210115
Issue:
Page Number:		104777 -
DOI:		10.1016/j.pestbp.2021.104777
ISSN/ISBN:		1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:		"Ceratocystis fimbriata is the pathogen of black rot disease, which widely exists in sweet potato producing areas all over the world. The antifungal activity of volatile organic compounds (VOCs) released by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 against C. fimbriata was reported in our previous study. In this study, we attempted to reveal the underlying antifungal mechanism of SPS-41 volatiles. Our results showed that the VOCs released by SPS-41 caused the morphological change of hyphae, destroyed the integrity of cell membrane, reduced the content of ergosterol, and induced massive accumulation of reactive oxygen species in C. fimbriata cells. Furthermore, SPS-41 fumigation decreased the mitochondrial membrane potential, acetyl-CoA and pyruvate content of C. fimbriata cells, as well as the mitochondrial dehydrogenases activity. In addition, the VOCs generated by SPS-41 reduced the intracellular ATP content and increased the extracellular ATP content of C. fimbriata. In summary, SPS-41 fumigation exerted its antifungal activity by inducing oxidative stress and mitochondrial dysfunction in C. fimbriata"
Keywords:		Antifungal Agents/pharmacology *Ascomycota Ceratocystis Mitochondria Oxidative Stress Plant Diseases Pseudomonas *Volatile Organic Compounds/pharmacology Antifungal Fumigation Membrane Mitochondrial dysfunction Reactive oxygen species;
Notes:		"MedlineZhang, Yu Li, Tengjie Xu, Mingjie Guo, Jianheng Zhang, Chunmei Feng, Zhaozhong Peng, Xue Li, Zongyun Xing, Ke Qin, Sheng eng 2021/03/28 Pestic Biochem Physiol. 2021 Mar; 173:104777. doi: 10.1016/j.pestbp.2021.104777. Epub 2021 Jan 15"