| Title: | Behavior and Electrophysiological Response of Gravid and Non-Gravid Lucilia cuprina (Diptera: Calliphoridae) to Carrion-Associated Compounds |
| Author(s): | Yan G; Liu S; Schlink AC; Flematti GR; Brodie BS; Bohman B; Greeff JC; Vercoe PE; Hu J; Martin GB; |
| Address: | "UWA Institute of Agriculture, University of Western Australia, Crawley, WA, Australia. Northwest Agriculture and Forestry University, College of Animal Science and Technology, Yangling, China. Department of Primary Industry and Regional Development, Livestock Industries, Agriculture and Food, South Perth, WA, Australia. School of Molecular Sciences, University of Western Australia, Crawley, WA, Australia. Department of Biological Sciences, Ohio University, Athens, OH" |
| ISSN/ISBN: | 1938-291X (Electronic) 0022-0493 (Linking) |
| Abstract: | "The Australian blow fly, Lucilia cuprina Wiedmann (Diptera: Calliphoridae), is a major cause of myiasis (flystrike) in Merino sheep in Australia and New Zealand and, as a primary colonizer of fresh carrion, also an important species in forensic investigations. Olfaction is considered the most important cue for insects to rapidly locate carrion over long distances, so the first carrion visitors are predicted to be very sensitive to carrion-related volatile compounds. We studied the responses of the Australian blow fly, Lucilia cuprina, to the carrion-associated compounds dimethyl trisulfide (DMTS), butyric acid, 1-octen-3-ol and indole. We also tested 2-mercaptoethanol, a compound commonly used in fly traps in Australia. We investigated whether responses of the flies are affected by their ovarian status by comparing responses of gravid and non-gravid L. cuprina in electroantennography (EAG) and two-choice laboratory bioassays. All four compounds evoked an EAG response, while only DMTS evoked responses in gas chromatography-mass spectrometry electroantennographic detection (GCMS-EAD) analyses and two-choice bioassays. Gravid flies detected lower doses of the test compounds than non-gravid flies. Our results indicate that DMTS is an important semiochemical for L. cuprina to locate carrion resources, and has potential for use in fly traps for flystrike control. Our observations also suggest that the greater sensitivity of gravid L. cuprina allows them to find fresh carrion quickly to maximize reproductive success by avoiding unsuitable degraded carrion" |
| Keywords: | Animals Butyric Acid/analysis Cadaver *Chemotaxis Diptera/*physiology Electrophysiological Phenomena Female Indoles/analysis Mercaptoethanol/analysis Octanols/analysis Odorants/*analysis *Olfactory Perception Sheep *Smell Sulfides/analysis; |
| Notes: | "MedlineYan, Guanjie Liu, Shimin Schlink, Anthony C Flematti, Gavin R Brodie, Bekka S Bohman, Bjorn Greeff, Johan C Vercoe, Philip E Hu, Jianhong Martin, Graeme B eng England 2018/08/08 J Econ Entomol. 2018 Aug 3; 111(4):1958-1965. doi: 10.1093/jee/toy115" |
