| Title: | Antifungal Activity of Bioactive Metabolites Produced by Trichoderma asperellum and Trichoderma atroviride in Liquid Medium |
| Author(s): | Stracquadanio C; Quiles JM; Meca G; Cacciola SO; |
| Address: | "Department of Agricultural Science, Mediterranean University of Reggio Calabria, Localita Feo di Vito, 89122 Reggio Calabria, Italy. Department of Agriculture, Food and Environment, University of Catania, Via S. Sofia 100, 95123 Catania, Italy. Department of Preventive Medicine, University of Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "Trichoderma spp. are known as biocontrol agents of fungal plant pathogens and have been recognized as a potential source of bioactive metabolites. The production of antimicrobial substances from strains T. atroviride (TS) and T. asperellum (IMI 393899) was investigated. The bioactivity of 10- and 30-day culture filtrate extracted with ethyl acetate was assessed against a set of pathogenic fungi and oomycetes. The 30-day extracts of both strains had significant cytotoxic effects against the tested pathogens, with values of minimum fungicidal concentration (MFC) ranging between 0.19 and 6.25 mg/mL. Dual culture assay (direct contact and nondirect contact) and the percentage inhibition of radial growth (PIRG) was calculated. The highest PIRG values were 76% and 81% (direct contact) with IMI 393899 and TS, respectively. Nondirect contact does not show inhibition on any of pathogens tested, indicating that the inhibition is not due to the secretion of volatile substances. Culture filtrates were analyzed by GC-MS and HPLC-Q-TOF-MS for the identification of volatile organic compounds (VOCs) and nonvolatile organic compounds (nVOCs), respectively. Seven classes of VOCs and 12 molecules of nVOCs were identified. These results indicate that these strains of Trichoderma had antimicrobial activities and they are potential natural sources of compounds with biological activity" |
| Keywords: | Trichoderma asperellum Trichoderma atroviride bioactive metabolites biological control plant pathogens; |
| Notes: | "PubMed-not-MEDLINEStracquadanio, Claudia Quiles, Juan Manuel Meca, Giuseppe Cacciola, Santa Olga eng 5A725192051/MIUR-FFABR 2017/ 5A722192134/project DIFESA/ PID2019-108070RB-100/Ministerio de Ciencia e Innovacion/ Prometeo/2018/126/Generalitat Valenciana/ Switzerland 2020/11/04 J Fungi (Basel). 2020 Nov 1; 6(4):263. doi: 10.3390/jof6040263" |
